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Product Info Summary
| SKU: | M00632 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DKK1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00632
BM4554 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DKK1 Rabbit Monoclonal Antibody catalog # M00632. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DKK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00632)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FHG-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DKK1
Reactive Species
M00632 is reactive to DKK1 in Human, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
28.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00632 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
IHC: human placenta tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human placenta, using DKK1 Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of DKK1 expression in HeLa cell lysate.
Specific Publications For Anti-DKK1 Rabbit Monoclonal Antibody (M00632)
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5 Customer Q&As for Anti-DKK1 Rabbit Monoclonal Antibody
Question
We have seen staining in rat decidua. Are there any suggestions? Is anti-DKK1 Rabbit Monoclonal antibody supposed to stain decidua positively?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Based on literature decidua does express DKK1. Based on Uniprot.org, DKK1 is expressed in decidua, leiomyosarcoma, fetal kidney, cerebellum, brain, among other tissues. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463
Boster Scientific Support
Answered: 2019-11-06
Question
We ordered your anti-DKK1 Rabbit Monoclonal antibody for WB on fetal kidney last year. I am using rat, and I plan to use the antibody for IF next. I am interested in examining fetal kidney as well as leiomyosarcoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I have checked the website and datasheets of our anti-DKK1 Rabbit Monoclonal antibody and it appears that M00632 has been tested on rat in both WB and IF. Thus M00632 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-16
Question
My colleagues were content with the WB result of your anti-DKK1 Rabbit Monoclonal antibody. However we have observed positive staining in decidua secreted. using this antibody. Is that expected? Could you tell me where is DKK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
From literature, decidua does express DKK1. Generally DKK1 expresses in secreted. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463
Boster Scientific Support
Answered: 2019-09-26
Question
We are currently using anti-DKK1 Rabbit Monoclonal antibody M00632 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-DKK1 Rabbit Monoclonal antibody (M00632) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
We are interested in using your anti-DKK1 Rabbit Monoclonal antibody for negative regulation of bmp signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
We appreciate your inquiry. This M00632 anti-DKK1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-11-13
