Anti-DKK1 Rabbit Monoclonal Antibody

Boster Bio Anti-DKK1 Rabbit Monoclonal Antibody catalog # M00632. Tested in IF, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: M00632
Size: 100 μl
Reactive Species: Human, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-DKK1 Rabbit Monoclonal Antibody

See all Dkk-1 products

SKU/Catalog Number

M00632

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DKK1 Rabbit Monoclonal Antibody catalog # M00632. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DKK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00632)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FHG-4

Immunogen

A synthesized peptide derived from human DKK1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00632 is reactive to DKK1 in Human, Rat

Applications

M00632 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Flow Cytometry 1:50

Validation Images & Assay Conditions

Gene/Protein Information For DKK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O94907

Gene ID

22943

Gene Name

DKK1

Full Name

Dickkopf-related protein 1

Weight

28.672kDa

Superfamily

dickkopf family

Alternative Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like DKK1 DKK-1, SK dickkopf WNT signaling pathway inhibitor 1 dickkopf-related protein 1|dickkopf 1 homolog|dickkopf-1 like|dickkopf-like protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DKK1, check out the DKK1 Infographic

DKK1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DKK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M00632

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-DKK1 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-DKK1 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-DKK1 Rabbit Monoclonal Antibody

Question

We have seen staining in rat decidua. Are there any suggestions? Is anti-DKK1 Rabbit Monoclonal antibody supposed to stain decidua positively?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

Based on literature decidua does express DKK1. Based on Uniprot.org, DKK1 is expressed in decidua, leiomyosarcoma, fetal kidney, cerebellum, brain, among other tissues. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463

Boster Scientific Support

Answered: 2019-11-06

Question

We ordered your anti-DKK1 Rabbit Monoclonal antibody for WB on fetal kidney last year. I am using rat, and I plan to use the antibody for IF next. I am interested in examining fetal kidney as well as leiomyosarcoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

I have checked the website and datasheets of our anti-DKK1 Rabbit Monoclonal antibody and it appears that M00632 has been tested on rat in both WB and IF. Thus M00632 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-16

Question

My colleagues were content with the WB result of your anti-DKK1 Rabbit Monoclonal antibody. However we have observed positive staining in decidua secreted. using this antibody. Is that expected? Could you tell me where is DKK1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

From literature, decidua does express DKK1. Generally DKK1 expresses in secreted. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463

Boster Scientific Support

Answered: 2019-09-26

Question

We are currently using anti-DKK1 Rabbit Monoclonal antibody M00632 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

The anti-DKK1 Rabbit Monoclonal antibody (M00632) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-08

Question

We are interested in using your anti-DKK1 Rabbit Monoclonal antibody for negative regulation of bmp signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

We appreciate your inquiry. This M00632 anti-DKK1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-11-13

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00632
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.