Anti-DMC1 Antibody Picoband™

Product Name

Anti-DMC1 Antibody Picoband™

View all DMC1 Antibodies

SKU/Catalog Number

A02978-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DMC1 Antibody Picoband™ catalog # A02978-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DMC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02978-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DMC1 recombinant protein (Position: D36-D283).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02978-3 is reactive to DMC1 in Human, Mouse, Rat

Applications

A02978-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

37.681kDa

Background of DMC1

Meiotic recombination protein DMC1/LIM15 homolog is a protein that in humans is encoded by the DMC1 gene. This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DMC1 using anti-DMC1 antibody (A02978-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMC1 antigen affinity purified polyclonal antibody (Catalog # A02978-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMC1 at approximately 37 kDa. The expected band size for DMC1 is at 37 kDa.

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Figure 2. IHC analysis of DMC1 using anti-DMC1 antibody (A02978-3).
DMC1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMC1 Antibody (A02978-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of DMC1 using anti-DMC1 antibody (A02978-3).
DMC1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMC1 Antibody (A02978-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of Jurkat cells using anti-DMC1 antibody (A02978-3).
Overlay histogram showing Jurkat cells stained with A02978-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DMC1 Antibody (A02978-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For DMC1 (Source: Uniprot.org, NCBI)

Alternative Names

disrupted meiotic cDNA1, yeast, homolog of; DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast); DMC1 homologue; HsLim15; LIM15yeast homolog) meiosis-specific homologousrecombination; meiotic recombination protein DMC1/LIM15 homolog; MGC150472; MGC150473 DMC1 DMC1H, LIM15, dJ199H16.1 DNA meiotic recombinase 1 meiotic recombination protein DMC1/LIM15 homolog|DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination|disrupted meiotic cDNA1, yeast, homolog of

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