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DMRT1 antibody (AZQ71MM5) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Zebrafish DMRT1 using anti-Zebrafish DMRT1 antibody (AZQ71MM5).
Zebrafish DMRT1 was detected in paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish DMRT1 Antibody (AZQ71MM5) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Zebrafish DMRT1 was detected in paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish DMRT1 Antibody (AZQ71MM5) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish DMRT1 Antibody Picoband®
DMRT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
DMRT1 antibody was positively detected by IHC in:
zebrafish ovary tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Zebrafish
Tested
Recommend
Human
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Zebrafish DMRT1 Antibody Picoband®
View all DMRT1 Antibodies
View all DMRT1 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20～C for one year from date of receipt.
After reconstitution, at 4～C for one month. It can also be aliquotted and stored frozen at -20～C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish DMRT1 recombinant protein (Position: R44-E76).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of DMRT1
Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.
Cite This Product
Anti-Zebrafish DMRT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ71MM5)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
94%
High Homology
Mouse
94%
High Homology
Rat
94%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Monkey
94%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for DMRT1 antibody
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Product Promise
We promise Anti-Zebrafish DMRT1 Antibody Picoband® performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ71MM5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026