Click image to see more details
Product Info Summary
| SKU: | AZQ71MM5 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Zebrafish DMRT1 Antibody Picoband®
SKU/Catalog Number
AZQ71MM5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish DMRT1 Antibody Picoband® catalog # AZQ71MM5. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Immunohistochemistry applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20～C for one year from date of receipt. After reconstitution, at 4～C for one month. It can also be aliquotted and stored frozen at -20～C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish DMRT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ71MM5)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish DMRT1 recombinant protein (Position: R44-E76).
Reactive Species
AZQ71MM5 is reactive to DMRT1 in Zebrafish
Background of DMRT1
Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ71MM5 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Positive Control
IHC: zebrafish ovary tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of Zebrafish DMRT1 using anti-Zebrafish DMRT1 antibody (AZQ71MM5).
Zebrafish DMRT1 was detected in paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish DMRT1 Antibody (AZQ71MM5) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish DMRT1 Antibody Picoband® (AZQ71MM5)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Zebrafish DMRT1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Zebrafish DMRT1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
