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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, IF, IHC, ICC, WB|
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Anti-DNA Polymerase beta Monoclonal Antibody
Boster Bio Anti-DNA Polymerase beta Monoclonal Antibody catalog # M01946. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Polymerase beta Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01946)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01946 is reactive to POLB in Human, Mouse, Rat
M01946 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POLB expression in A431 cell lysate using anti-POLB antibody (M01946).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLB antigen affinity purified polyclonal antibody (Catalog # M01946) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for POLB.
Gene/Protein Information For POLB (Source: Uniprot.Org, NCBI)
DNA polymerase beta
DNA polymerase type-X family
DNA pol beta; DNA polymerase beta subunit; DNA Polymerase beta; EC 220.127.116.11; EC 4.2.99.-; MGC125976; POLB; polymerase (DNA directed), beta POLB DNA polymerase beta DNA polymerase beta|DNA pol beta|DNA polymerase beta subunit|polymerase (DNA directed), beta|polymerase (DNA) beta*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on POLB, check out the POLB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for POLB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M01946
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