Anti-DNA Polymerase beta Monoclonal Antibody

Boster Bio Anti-DNA Polymerase beta Monoclonal Antibody catalog # M01946. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01946
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-DNA Polymerase beta Monoclonal Antibody

See all DNA Polymerase beta products

SKU/Catalog Number

M01946

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DNA Polymerase beta Monoclonal Antibody catalog # M01946. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DNA Polymerase beta Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01946)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACGH-16

Immunogen

A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01946 is reactive to POLB in Human, Mouse, Rat

Applications

M01946 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For POLB (Source: Uniprot.Org, NCBI)

Uniprot ID

P06746

Gene ID

5423

Gene Name

POLB

Full Name

DNA polymerase beta

Weight

38.178kDa

Superfamily

DNA polymerase type-X family

Alternative Names

DNA pol beta; DNA polymerase beta subunit; DNA Polymerase beta; EC 2.7.7.7; EC 4.2.99.-; MGC125976; POLB; polymerase (DNA directed), beta POLB DNA polymerase beta DNA polymerase beta|DNA pol beta|DNA polymerase beta subunit|polymerase (DNA directed), beta|polymerase (DNA) beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on POLB, check out the POLB Infographic

POLB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for POLB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01946

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5 Customer Q&As for Anti-DNA Polymerase beta Monoclonal Antibody

Question

What sample was tested in WB and IHC applications with M01946?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of DNA Polymerase beta expression in A431 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

Is this M01946 anti-DNA Polymerase beta Monoclonal antibody reactive to the isotypes of POLB?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

The immunogen of M01946 anti-DNA Polymerase beta Monoclonal antibody is A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-24

Question

Please see the WB image, lot number and protocol we used for testis using anti-DNA Polymerase beta Monoclonal antibody M01946. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-02-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-27

Question

My question regarding product M01946, anti-DNA Polymerase beta Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Krishna

Verified customer

Asked: 2018-12-26

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01946 anti-DNA Polymerase beta Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-26

Question

I was wanting to use your anti-DNA Polymerase beta Monoclonal antibody for WB for rat testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat testis identification?

L. Williams

Verified customer

Asked: 2016-06-20

Answer

You can see on the product datasheet, M01946 anti-DNA Polymerase beta Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-06-20

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M01946
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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