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Product Info Summary
| SKU: | M01946 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DNA Polymerase beta Monoclonal Antibody
SKU/Catalog Number
M01946
BM5317 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DNA Polymerase beta Monoclonal Antibody catalog # M01946. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Polymerase beta Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01946)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACGH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Reactive Species
M01946 is reactive to POLB in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
38.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01946 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of DNA Polymerase beta expression in A431 cell lysate.
Specific Publications For Anti-DNA Polymerase beta Monoclonal Antibody (M01946)
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5 Customer Q&As for Anti-DNA Polymerase beta Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01946?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of DNA Polymerase beta expression in A431 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this M01946 anti-DNA Polymerase beta Monoclonal antibody reactive to the isotypes of POLB?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The immunogen of M01946 anti-DNA Polymerase beta Monoclonal antibody is A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-24
Question
Please see the WB image, lot number and protocol we used for testis using anti-DNA Polymerase beta Monoclonal antibody M01946. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-27
Question
My question regarding product M01946, anti-DNA Polymerase beta Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Krishna
Verified customer
Asked: 2018-12-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01946 anti-DNA Polymerase beta Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-26
Question
I was wanting to use your anti-DNA Polymerase beta Monoclonal antibody for WB for rat testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat testis identification?
L. Williams
Verified customer
Asked: 2016-06-20
Answer
You can see on the product datasheet, M01946 anti-DNA Polymerase beta Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-20
