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- Table of Contents
Facts about DNA polymerase beta.
Conducts'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. .
Human | |
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Gene Name: | POLB |
Uniprot: | P06746 |
Entrez: | 5423 |
Belongs to: |
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DNA polymerase type-X family |
DNA pol beta; DNA polymerase beta subunit; DNA Polymerase beta; EC 2.7.7.7; EC 4.2.99.-; MGC125976; POLB; polymerase (DNA directed), beta
Mass (kDA):
38.178 kDA
Human | |
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Location: | 8p11.21 |
Sequence: | 8; NC_000008.11 (42338452..42371810) |
Nucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
Antibodies against DNA polymerase beta (POLB) are available at Boster Bio. In this article we will discuss its validation, applications, and citations. Boster also offers a variety of high-affinity secondary antibodies. These antibodies are trusted by the research community and have been validated on Western Blotting, Immunohistochemistry, and ELISA. As you can see, these antibodies are a valuable tool for your research.
Boster Bio's anti-DNA polymerase beta (POLB) antibody has been tested for ICC, IHC, and IP applications. It reacts with human, mouse, and rat DNA polymerase. Moreover, this antibody has been used in studies in zebrafish. The results of these studies suggest that this antibody can help researchers analyze DNA polymerase activity in a variety of species.
The validation of the POLB Marker is a critical step in QTL studies. The SSBR pathway involves base excision repair, which requires POLB. In addition to its role in DNA polymerase function, POLB is also a lyase. The resulting deletion of POLB resulted in embryonic lethality in mice. Interestingly, PolbNes-Cre animals had comparable lifespans to WT controls, while Xrcc1Nes-Cre mice developed a shorter lifespan. Both mice succumbed to medulloblastoma.
The validation of the POLB Marker required a large number of SNPs and amplification of the target E. coli strain. Twelve primer sets were developed to detect the target strains. These primer sets were selected on the basis of abundant SNP sites in the genome aligned WGS of eleven genes. Another study by Grish and Burbudae identified nine candidate markers. The chosen primer sets used natural and artificial SNPs, and were mismatched within three bases at 3' end SNP sites.
The POLB marker is a polyclonal antibody raised against POLB polymerase, a protein involved in base excision and gap-filling DNA synthesis. It normally lives in the cytoplasm, but upon DNA damage, translocates into the nucleus. POLB siRNA recognizes amino acids 1-335. It is shipped at 4degC for maximum stability. The corresponding human POLB protein is provided for each lot.
The POLB gene sequence consists of three SNPs. These three are natural and the fourth is artificial. In O.ileS2-1F, a SNP is present in three bases: a blue base changed to G and two underlined nucleotide positions. The POLB-4-F primer does not contain an altered artificial base. The SNP-based primers improved the ability to bind to PCR templates and detect allele-specific DNA.
The company has appointed LAB-A-PORTER Limited as an official distributor of Boster Bio products. Boster Bio has been manufacturing high-quality antibodies and ELISA kits since 1993. These reagents have received over 29,000 citations. The company's website facilitates a faster, more transparent and cost-effective product selection journey. The company's Bioz Badges and Citations in Boster Bio help researchers identify highly regarded Boster Bio products.
Boster Biologicals develops ELISA kits and research antibodies. These kits detect biomarkers in various fields, including neuroscience, cancer, and development biology. They have picogram-level sensitivity. Additionally, Boster Bio sells immunological reagents through tebu-bio. They also offer highly sensitive ELISA kits and IHC-ready antibodies. The company's quality assurance is backed by a Boster Quality Guarantee.
PMID: 10648175 by Patterson T.A., et al. Molecular cloning and high-level expression of human polymerase beta cDNA and comparison of the purified recombinant human and rat enzymes.
PMID: 7705833 by Dobashi Y., et al. Polymorphisms in the human DNA polymerase beta gene.