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Product Info Summary
| SKU: | M00319 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
SKU/Catalog Number
M00319
BM3912 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody catalog # M00319. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00319)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FO-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Dnmt3b
Reactive Species
M00319 is reactive to DNMT3B in Human, Mouse, Rat
Observed Molecular Weight
96 kDa
Calculated molecular weight
95.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00319 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of Dnmt3b expression in (1) A549 cell lysate; (2) A431 cell lysate.
Specific Publications For Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody (M00319)
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4 Customer Q&As for Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
Question
We are currently using anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody (M00319) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-10
Question
I was wanting to use your anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody for WB for human secondary oocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human secondary oocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
As indicated on the product datasheet, M00319 anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human secondary oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
I am interested in to test anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 on human secondary oocyte for research purposes, then I may be interested in using anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
The products we sell, including anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-22
Question
Does anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 work on horse ICC with fetal testis small intestine?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
Our lab technicians have not tested anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Dnmt3b Rabbit Monoclonal antibody Rabbit Monoclonal antibody M00319 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse fetal testis small intestine in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-29
