Anti-DNMT3L Antibody

Product Name

Anti-DNMT3L Antibody

View all DNMT3L Antibodies

SKU/Catalog Number

A04214

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-DNMT3L Antibody (Catalog # A04214). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-DNMT3L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04214)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04214 is reactive to DNMT3L in Human

Applications

A04214 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

43.583kDa

Background of DNMT3L

DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1000 - 1:3000
IHC: 1:500
IP: 1:100
WB: 1:500

Validation Images & Assay Conditions

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DNMT3L was detected in paraffin-embedded sections of human testis tissues using rabbit anti-DNMT3L Antigen Affinity purified polyclonal antibody (Catalog # A04214) at 1:500. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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Western Blot and immunoprecipitation of rabbit anti-DNMT3L antibody. DNMT3L at 43.6KD was detected using rabbit anti-DNMT3L Antigen Affinity purified polyclonal antibody (Catalog # A04214 at 1:1500. Transfection of U2OS cells (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. This antibody works for western blotting only following immunoprecipitation.

Gene/Protein Information For DNMT3L (Source: Uniprot.org, NCBI)

Alternative Names

DNA (cytosine-5-)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein; MGC1090 DNMT3L DNA methyltransferase 3 like DNA (cytosine-5)-methyltransferase 3-like|DNA (cytosine-5-)-methyltransferase 3-like|cytosine-5-methyltransferase 3-like protein|human cytosine-5-methyltransferase 3-like protein

For more info on DNMT3L, check out the DNMT3L Infographic

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In this infographic, you will see the following information for DNMT3L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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