Anti-Docking protein 3 DOK3 Antibody

Dok3 antibody

Boster Bio Anti-Docking protein 3 DOK3 Antibody (Catalog # A09019-1). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A09019-1
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Docking protein 3 DOK3 Antibody

View all Dok3 Antibodies

SKU/Catalog Number

A09019-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Docking protein 3 DOK3 Antibody (Catalog # A09019-1). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Docking protein 3 DOK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09019-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human DOK3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09019-1 is reactive to Dok3 in Human, Mouse

Applications

A09019-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

48.027kDa

Background of Dok3

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Favre C., Genes Immun. 4:40-45(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Dok3 (Source: Uniprot.org, NCBI)

Gene Name

Dok3

Full Name

Docking protein 3

Weight

48.027kDa

Superfamily

DOK family

Alternative Names

docking protein 3; DOK3; DOKL; Dok-like protein; Downstream of tyrosine kinase 3; FLJ22570; FLJ39939 Dok3|AI450713, Dokl|docking protein 3|docking protein 3|DOK-L|Dok-like protein|downstream of tyrosine kinase 3|p62(dok)-like protein|p62Dok-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Dok3, check out the Dok3 Infographic

Dok3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Dok3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Docking protein 3 DOK3 Antibody

Question

We are currently using anti-DOK3 antibody A09019-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The anti-DOK3 antibody (A09019-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-24

Question

I am looking for to test anti-DOK3 antibody A09019-1 on human erythroleukemia for research purposes, then I may be interested in using anti-DOK3 antibody A09019-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

The products we sell, including anti-DOK3 antibody A09019-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-07

Question

Will anti-DOK3 antibody A09019-1 work on bovine WB with blood?

R. Johnson

Verified customer

Asked: 2015-08-07

Answer

Our lab technicians have not validated anti-DOK3 antibody A09019-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-DOK3 antibody A09019-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-08-07

Question

I see that the anti-DOK3 antibody A09019-1 works with WB, what is the protocol used to produce the result images on the product page?

S. Anderson

Verified customer

Asked: 2014-10-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-10-28

Question

I have a question about product A09019-1, anti-DOK3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Zhang

Verified customer

Asked: 2014-08-27

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09019-1 anti-DOK3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-08-27

Order DetailsPrice
A09019-1-100uL

100uL

$360

Free Secondary Antibody

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A09019-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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