Anti-Dopamine Receptor D₂ Drd2 Antibody

Product Name

Anti-Dopamine Receptor D₂ Drd2 Antibody

View all Dopamine D2R/DRD2 Antibodies

SKU/Catalog Number

A00289

Size

100µg

Form

Liquid

Description

Boster Bio Anti-Dopamine Receptor D₂ Drd2 Antibody (Catalog# A00289). Tested in ELISA, IHC, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for long-term storage. Keep on ice at all times.

Cite This Product

Anti-Dopamine Receptor D₂ Drd2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00289)

Host

Rabbit

Contents

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Clonality

Polyclonal

Clone Number

CFA-18

Isotype

Rabbit IgG

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus of mouse dopamine receptor D₂

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Predicted species reactivity based on epitope sequence homology: rhesus macaque, chimpanzee, horse, arctic fox, dog, opossum, ferret.

Reactive Species

A00289 is reactive to DRD2 in Human, Mouse, Rat

Applications

A00289 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.619kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~51kDa.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Cellular ELISA: HEK293 cells expressing dopamine receptor (D2) were treated with indicated concentrations of agonist (Quinpirol) and antagonist (Siperone) and probed with Dopamine Receptor (D2) pAb (1:1000 of a stock solution of 1μg/μL) by cellular ELISA.Data from vehicle treated cells were taken as 100%.Results are the mean ±SEM (n=3).

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Figure 1. Western blot analysis of Drd2 using anti-Drd2 antibody (A00289).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Drd2 antigen affinity purified polyclonal antibody (Catalog # A00289) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

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Figure 2. IHC analysis of Drd2 using anti-Drd2 antibody (A00289).
Drd2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Drd2 Antibody (A00289) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For DRD2 (Source: Uniprot.org, NCBI)

Alternative Names

D(2) dopamine receptor; D2DR; D2R; Dopamine D2 R; Dopamine D2 receptor; Dopamine D2R; dopamine receptor D2 isoform; dopamine receptor D2; DRD2; seven transmembrane helix receptor DRD2 D2DR, D2R dopamine receptor D2 D(2) dopamine receptor|dopamine D2 receptor|seven transmembrane helix receptor

For more info on DRD2, check out the DRD2 Infographic

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In this infographic, you will see the following information for DRD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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