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Product Info Summary
| SKU: | A00840 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DOT1L Antibody
SKU/Catalog Number
A00840
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DOT1L Antibody catalog # A00840. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DOT1L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00840)
Host
Rabbit
Contents
Liquid form in PBS containing 50% glycerol,and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human DOT1L protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00840 is reactive to DOT1L in Human
Applications
A00840 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
164.856kDa
Background of DOT1L
Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000 ELISA 1:5000-20000
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For DOT1L (Source: Uniprot.org, NCBI)
Gene Name
DOT1L
Full Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Weight
164.856kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
DOT1L; DOT1-like protein; DOT1-like, histone H3 methyltransferase (S. cerevisiae); EC 2.1.1.43; H3-K79-HMTase; KIAA1814DKFZp586P1823; KMT4; KMT4H3 lysine-79 specific; Lysine N-methyltransferase 4 DOT1L DOT1, KMT4 DOT1 like histone lysine methyltransferase histone-lysine N-methyltransferase, H3 lysine-79 specific|DOT1 like histone H3K79 methyltransferase|DOT1-like histone methyltransferase|DOT1-like protein|DOT1-like, histone H3 methyltransferase|H3-K79-HMTase|histone H3-K79 methyltransferase|histone methyltransferase DOT1L|lysine N-methyltransferase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOT1L, check out the DOT1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOT1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DOT1L Antibody (A00840)
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No publications found for A00840
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Recommended Resources
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Customer Reviews
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