Anti-Doublecortin/DCX Monoclonal Antibody

Boster Bio Anti-Doublecortin/DCX Monoclonal Antibody catalog # M01053-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01053-1
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IF, IHC, ICC, WB

Product Name

Anti-Doublecortin/DCX Monoclonal Antibody

See all Doublecortin products

SKU/Catalog Number

M01053-1

Size

100ul

Description

Boster Bio Anti-Doublecortin/DCX Monoclonal Antibody catalog # M01053-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Doublecortin/DCX Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01053-1)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

Clonality

Monoclonal

Immunogen

Recombinant full length human Lis-A isoform of DCX purified from E. coli.

Reactive Species

M01053-1 is reactive to DCX in Human, Mouse, Rat

Applications

M01053-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:10,000. IF/ICC and IHC: 1:1,000.

Validation Images & Assay Conditions

Gene/Protein Information For DCX (Source: Uniprot.Org, NCBI)

Uniprot ID

O43602

Gene ID

1641

Gene Name

DCX

Full Name

Neuronal migration protein doublecortin

Weight

40.574kDa

Alternative Names

DBCN; Doublecortex; Doublecortin; Doublin; FLJ51296; Lissencephalin-X; LISX; Lis-X; X-linked (doublecortin) DCX DBCN, DC, LISX, SCLH, XLIS doublecortin neuronal migration protein doublecortin|doublecortex|doublin|lis-X|lissencephalin-X

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DCX, check out the DCX Infographic

DCX infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DCX: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01053-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang Xm, Du F, Yang D, Yu Cj, Huang Xn, Liu W, Fu J. Bmc Neurosci. 2010 Oct 25;11:138. Doi: 10.1186/1471-2202-11-138. Transplanted Bone Marrow Stem Cells Relocate To Infarct Penumbra And Co-Express Endogenous Proliferative And Immature Neuronal M...

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16 Customer Q&As for Anti-Doublecortin/DCX Monoclonal Antibody

Question

My question regarding product M01053-1, anti-Doublecortin/DCX Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01053-1 anti-Doublecortin/DCX Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-25

Question

I see that the anti-Doublecortin/DCX Monoclonal antibody M01053-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-29

Question

Is a blocking peptide available for product anti-Doublecortin/DCX Monoclonal antibody (M01053-1)?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

We do provide the blocking peptide for product anti-Doublecortin/DCX Monoclonal antibody (M01053-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-01

Question

We are currently using anti-Doublecortin/DCX Monoclonal antibody M01053-1 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

The anti-Doublecortin/DCX Monoclonal antibody (M01053-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-29

Question

you antibody using your anti-Doublecortin/DCX Monoclonal antibody for x-linked 1 (lisx1) studies. Has this antibody been tested with western blotting on developing neuronal cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

I appreciate your inquiry. This M01053-1 anti-Doublecortin/DCX Monoclonal antibody is tested on developing neuronal cells. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-20

Question

Will anti-Doublecortin/DCX Monoclonal antibody M01053-1 work for WB with brain?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

According to the expression profile of brain, DCX is highly expressed in brain. So, it is likely that anti-Doublecortin/DCX Monoclonal antibody M01053-1 will work for WB with brain.

Boster Scientific Support

Answered: 2019-08-05

Question

Our lab want to know about to test anti-Doublecortin/DCX Monoclonal antibody M01053-1 on mouse brain for research purposes, then I may be interested in using anti-Doublecortin/DCX Monoclonal antibody M01053-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The products we sell, including anti-Doublecortin/DCX Monoclonal antibody M01053-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-03

Question

Is this M01053-1 anti-Doublecortin/DCX Monoclonal antibody reactive to the isotypes of DCX?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

The immunogen of M01053-1 anti-Doublecortin/DCX Monoclonal antibody is Recombinant full length human Lis-A isoform of DCX purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-29

Question

Please see the WB image, lot number and protocol we used for brain using anti-Doublecortin/DCX Monoclonal antibody M01053-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-17

Question

My colleagues were content with the WB result of your anti-Doublecortin/DCX Monoclonal antibody. However we have been able to see positive staining in brain cytoplasm. cell projection, using this antibody. Is that expected? Could you tell me where is DCX supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

From literature, brain does express DCX. Generally DCX expresses in cytoplasm. cell projection,. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651

Boster Scientific Support

Answered: 2019-03-11

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Doublecortin/DCX Monoclonal antibody M01053-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-09

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-09

Question

I was wanting to use your anti-Doublecortin/DCX Monoclonal antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?

Verified Customer

Verified customer

Asked: 2018-11-08

Answer

You can see on the product datasheet, M01053-1 anti-Doublecortin/DCX Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-08

Question

Is there a BSA free version of anti-Doublecortin/DCX Monoclonal antibody M01053-1 available?

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Doublecortin/DCX Monoclonal antibody M01053-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-18

Question

We bought anti-Doublecortin/DCX Monoclonal antibody for WB on fetal brain a few years ago. I am using rat, and We want to use the antibody for IHC next. I would like examining fetal brain as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2017-06-28

Answer

I looked at the website and datasheets of our anti-Doublecortin/DCX Monoclonal antibody and it seems that M01053-1 has been tested on rat in both WB and IHC. Thus M01053-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-06-28

Question

Does M01053-1 anti-Doublecortin/DCX Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

C. Baker

Verified customer

Asked: 2014-11-20

Answer

As indicated on the product datasheet, M01053-1 anti-Doublecortin/DCX Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-11-20

Question

We have seen staining in rat brain. What should we do? Is anti-Doublecortin/DCX Monoclonal antibody supposed to stain brain positively?

M. Dhar

Verified customer

Asked: 2013-08-02

Answer

From what I have seen in literature brain does express DCX. From what I have seen in Uniprot.org, DCX is expressed in frontal cortex, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651

Boster Scientific Support

Answered: 2013-08-02

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M01053-1
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