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Product Info Summary
| SKU: | A03227 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-DR3 TNFRSF25 Antibody
SKU/Catalog Number
A03227
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DR3 TNFRSF25 Antibody (Catalog # A03227). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DR3 TNFRSF25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03227)
Host
Rabbit
Contents
DR3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DR3 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human DR3. The immunogen is located within the last 50 amino acids of DR3.
Cross-reactivity
No cross reaction to other death receptors.
Reactive Species
A03227 is reactive to TNFRSF25 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
45.4 kDa
Background of TNFRSF25
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel cell death receptor has not yet been defined. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-κB activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03227 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DR3 antibody can be used for Western blot 0.5 μg/mL. A 59 kDa band should be detected.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:500 dilution.
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Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μg/mL.
Green: DR3 Antibody (A03227)
Blue: DAPI staining
Specific Publications For Anti-DR3 TNFRSF25 Antibody (A03227)
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