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Product Info Summary
| SKU: | A30480 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DUSP4 Antibody
SKU/Catalog Number
A30480
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DUSP4 Antibody (Catalog# A30480). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DUSP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30480)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DUSP4. Immunogen sequence location: 81-130
Reactive Species
A30480 is reactive to DUSP4 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
43.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30480 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HEPG2-UV cells using MKP-2 Polyclonal Antibody diluted at 1:1000.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DUSP4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from RAW264.7, A549, COS7, and NIH/3T3 cells, using DUSP4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of A549 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-DUSP4 Antibody (A30480)
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1 Customer Q&As for Anti-DUSP4 Antibody
Question
We are currently using anti-DUSP4 antibody A30480 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The anti-DUSP4 antibody (A30480) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-06
