Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody

Product Name

Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody

View all MKP-3/DUSP6 Antibodies

SKU/Catalog Number

M02157

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody catalog # M02157. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DUSP6/Mkp 3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02157)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBE-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DUSP6

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02157 is reactive to DUSP6 in Human, Mouse, Rat

Applications

M02157 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

42.32kDa

Background of MKP-3/DUSP6

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Western blot analysis of DUSP6 expression in NIH/3T3 cell lysate.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunohistochemical analysis of paraffin-embedded human thyroid, using DUSP6 Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For DUSP6 (Source: Uniprot.org, NCBI)

Alternative Names

dual specificity phosphatase 6; Dual specificity protein phosphatase PYST1; DUSP6; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP3; MKP-3; MKP3serine/threonine specific protein phosphatase; PYST1; PYST1dual specificity protein phosphatase 6 DUSP6 HH19, MKP3, PYST1 dual specificity phosphatase 6 dual specificity protein phosphatase 6|MAP kinase phosphatase 3|dual specificity protein phosphatase PYST1|mitogen-activated protein kinase phosphatase 3|serine/threonine specific protein phosphatase

For more info on DUSP6, check out the DUSP6 Infographic

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In this infographic, you will see the following information for DUSP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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