Anti-DUT Antibody

dUTPase antibody

Boster Bio Anti-DUT Antibody catalog # PA1030-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1030-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-DUT Antibody

View all dUTPase Antibodies

SKU/Catalog Number

PA1030-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DUT Antibody catalog # PA1030-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DUT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1030-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DUT, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1030-1 is reactive to DUT in Human, Mouse, Rat

Applications

PA1030-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

26.563kDa

Background of dUTPase

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E.coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For DUT (Source: Uniprot.org, NCBI)

Gene Name

DUT

Full Name

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Weight

26.563kDa

Superfamily

dUTPase family

Alternative Names

deoxyuridine triphosphatase; dUTP pyrophosphatasedUTP nucleotidohydrolase; dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; EC 3.6.1.23; FLJ20622 DUT dUTPase deoxyuridine triphosphatase deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial|dUTP diphosphatase|dUTP nucleotidohydrolase|dUTP pyrophosphatase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DUT, check out the DUT Infographic

DUT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DUT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-DUT Antibody

Question

We are currently using anti-DUT antibody PA1030-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

The anti-DUT antibody (PA1030-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-07

Question

I see that the anti-DUT antibody PA1030-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-17

Question

I was wanting to use your anti-DUT antibody for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

N. Collins

Verified customer

Asked: 2019-07-23

Answer

You can see on the product datasheet, PA1030-1 anti-DUT antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-23

Question

We want to test anti-DUT antibody PA1030-1 on human brain for research purposes, then I may be interested in using anti-DUT antibody PA1030-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-27

Answer

The products we sell, including anti-DUT antibody PA1030-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-27

Question

Is there a BSA free version of anti-DUT antibody PA1030-1 available?

K. Li

Verified customer

Asked: 2017-05-29

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-DUT antibody PA1030-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-05-29

Question

Will PA1030-1 anti-DUT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Parker

Verified customer

Asked: 2016-04-29

Answer

It shows on the product datasheet, PA1030-1 anti-DUT antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-04-29

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-DUT antibody PA1030-1. Let me know if you need anything else.

M. Zhao

Verified customer

Asked: 2015-09-22

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-09-22

Order DetailsPrice
PA1030-1

100μg

$370
PA1030-1-10ug

10μg sample (liquid)

$99
PA1030-1-Biotin

100 μg Biotin conjugated

$570
PA1030-1-Cy3

100 μg Cy3 conjugated

$570
PA1030-1-Dylight488

100 μg Dylight488 conjugated

$570
PA1030-1-Dylight550

100 μg Dylight550 conjugated

$570
PA1030-1-Dylight594

100 μg Dylight594 conjugated

$570
PA1030-1-FITC

100 μg FITC conjugated

$570
PA1030-1-HRP

100 μg HRP conjugated

$570
PA1030-1-APC

100 μg APC conjugated

$670
PA1030-1-PE

100 μg PE conjugated

$670
PA1030-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1030-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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