Anti-EBP1 Antibody (C-term)

Product Name

Anti-EBP1 Antibody (C-term)

View all PA2G4/EBP1 Antibodies

SKU/Catalog Number

A02791-2

Size

80 µl

Description

Boster Bio Anti-EBP1 Antibody (C-term) (Catalog # A02791-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-EBP1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02791-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human EBP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02791-2 is reactive to PA2G4 in Human, Mouse

Applications

A02791-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

43.787kDa

Background of PA2G4/EBP1

PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50

Validation Images & Assay Conditions

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EBP1 Antibody (C-term) western blot analysis in Jurkat,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the EBP1 antibody detected the EBP1 protein (arrow).

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All lanes : Anti-EBP1 Antibody (C-term) at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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EBP1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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EBP1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For PA2G4 (Source: Uniprot.org, NCBI)

Alternative Names

Cell cycle protein p38-2G4 homolog; EBP1; ErbB-3 binding protein 1; ErbB3-binding protein 1; ErbB3-binding protein Ebp1; HG4-1; p38-2G4; PA2G4; proliferation-associated 2G4, 38kD; proliferation-associated 2G4, 38kDa; proliferation-associated protein 2G4 PA2G4 EBP1, HG4-1, p38-2G4 proliferation-associated 2G4 proliferation-associated protein 2G4|ErbB-3 binding protein 1|ErbB3-binding protein Ebp1|cell cycle protein p38-2G4 homolog|erbB3-binding protein 1|proliferation-associated 2G4, 38kD|proliferation-associated 2G4, 38kDa

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