Product Info Summary
SKU: | M00830-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EEF2 Monoclonal Antibody
SKU/Catalog Number
M00830-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EEF2 Monoclonal Antibody catalog # M00830-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EEF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00830-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADBH-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EEF2 Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00830-1 is reactive to EEF2 in Human, Mouse, Rat
Applications
M00830-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
95.338kDa
Background of eEF-2
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of EEF2 expression in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For EEF2 (Source: Uniprot.org, NCBI)
Gene Name
EEF2
Full Name
Elongation factor 2
Weight
95.338kDa
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
eEF2; eEF-2; EF-2; EF2EEF-2; elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase EEF2 EEF-2, EF-2, EF2, SCA26 eukaryotic translation elongation factor 2 elongation factor 2|epididymis secretory sperm binding protein|polypeptidyl-tRNA translocase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EEF2, check out the EEF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EEF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EEF2 Monoclonal Antibody (M00830-1)
Hello CJ!
No publications found for M00830-1
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-EEF2 Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-EEF2 Monoclonal antibody M00830-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
Would M00830-1 anti-EEF2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
You can see on the product datasheet, M00830-1 anti-EEF2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-05
Question
Is a blocking peptide available for product anti-EEF2 Monoclonal antibody (M00830-1)?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We do provide the blocking peptide for product anti-EEF2 Monoclonal antibody (M00830-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-EEF2 Monoclonal antibody M00830-1 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The anti-EEF2 Monoclonal antibody (M00830-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-01
Question
I was wanting to use your anti-EEF2 Monoclonal antibody for IP for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
A. Jones
Verified customer
Asked: 2015-05-07
Answer
It shows on the product datasheet, M00830-1 anti-EEF2 Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-07
Question
Would anti-EEF2 Monoclonal antibody M00830-1 work on goat WB with liver?
M. Krishna
Verified customer
Asked: 2014-01-06
Answer
Our lab technicians have not validated anti-EEF2 Monoclonal antibody M00830-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-EEF2 Monoclonal antibody M00830-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-06