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Product Info Summary
| SKU: | M00830-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EEF2 Monoclonal Antibody
SKU/Catalog Number
M00830-1
BM5382 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EEF2 Monoclonal Antibody catalog # M00830-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EEF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00830-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADBH-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EEF2 Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
Reactive Species
M00830-1 is reactive to EEF2 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
95.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00830-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:50
Positive Control
WB: human 293T whole cell, human SiHa whole cell, human Jurkat whole cell, human A549 whole cell, human K562 whole cell, human MCF-7 whole cell, human HEL whole cell, human Caco-2 whole cell
IHC: human prostate cancer tissue, rat ovary tissue
Validation Images & Assay Conditions
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Western blot analysis of EEF2 using anti-EEF2 antibody (M00830-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: human HEL whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2 antigen affinity purified monoclonal antibody (Catalog # M00830-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEF2 at approximately 100 kDa. The expected band size for EEF2 is at 95 kDa.
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Western blot analysis of EEF2 expression in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.
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IHC analysis of EEF2 using anti-EEF2 antibody (M00830-1).
EEF2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF2 Antibody (M00830-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of EEF2 using anti-EEF2 antibody (M00830-1).
EEF2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF2 Antibody (M00830-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-EEF2 Monoclonal Antibody (M00830-1)
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6 Customer Q&As for Anti-EEF2 Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-EEF2 Monoclonal antibody M00830-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
Would M00830-1 anti-EEF2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
You can see on the product datasheet, M00830-1 anti-EEF2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-05
Question
Is a blocking peptide available for product anti-EEF2 Monoclonal antibody (M00830-1)?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We do provide the blocking peptide for product anti-EEF2 Monoclonal antibody (M00830-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-EEF2 Monoclonal antibody M00830-1 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The anti-EEF2 Monoclonal antibody (M00830-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-01
Question
I was wanting to use your anti-EEF2 Monoclonal antibody for IP for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
A. Jones
Verified customer
Asked: 2015-05-07
Answer
It shows on the product datasheet, M00830-1 anti-EEF2 Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-07
Question
Would anti-EEF2 Monoclonal antibody M00830-1 work on goat WB with liver?
M. Krishna
Verified customer
Asked: 2014-01-06
Answer
Our lab technicians have not validated anti-EEF2 Monoclonal antibody M00830-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-EEF2 Monoclonal antibody M00830-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-06
