Elongation factor 2 Antibodies

AND EEF2 ELISA kits, Elongation factor 2 Proteins

Many biological assays use antibodies to detect Elongation factor 2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Elongation factor 2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Elongation factor 2 antibodies...

We validate the specificity of these antibodies to Elongation factor 2 by testing them on tissues known to express EEF2 positively and negatively. Browse below to find the EEF2 antibody that suites your experiment. We have 22 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Elongation factor 2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the EEF2 Western Blot Guide

EEF2 Infographic by Boster Bio

All EEF2 Products

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Applications

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Anti-EEF2/Elongation factor 2 Antibody Picoband®

Western blot analysis of EEF2/Elongation factor 2 using anti-EEF2/Elongation factor 2 antibody (A00830-2)

IHC analysis of EEF2/Elongation factor 2 using anti-EEF2/Elongation factor 2 antibody (A00830-2)

+10

Human, Monkey, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A00830-2
100 μg/vial

Anti-EEF2 Antibody Picoband®

Western blot analysis of EEF2 using anti-EEF2 antibody (A00830-1)

IHC analysis of EEF2 using anti-EEF2 antibody (A00830-1)

Human, Monkey, Mouse, Rat, Zebrafish

ELISA, IHC, WB

$370
Cat # A00830-1
100 μg/vial

Anti-EEF2K Antibody Picoband®

Western blot analysis of EEF2K using anti-EEF2K antibody (A02277-3)

IF analysis of EEF2K using anti-EEF2K antibody (A02277-3)

Flow Cytometry analysis of SiHa cells using anti-EEF2K antibody (A02277-3)

Human, Rat

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A02277-3
100 μg/vial

Anti-EEF2 Picoband® Antibody (monoclonal, 5F5)

Western blot analysis of EEF2 using anti-EEF2 antibody (M00830-2)

IHC analysis of EEF2 using anti-EEF2 antibody (M00830-2)

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M00830-2
100 μg/vial

Anti-eEF2K (phospho-S366) Antibody

Western blot (WB) analysis of p-eEF2K (S366) pAb at 1:1000 dilution
Lane1:MCF-7 whole cell lysate(40ug)
Lane2:Hela whole cell lysate(40ug)
Lane3:A375 whole cell lysate(40ug)

Immunoprecipitation of HEK293T cell lysate using p-eEF2K (S366) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4)

Human

IP, IHC, WB

$482
Cat # A02277S366-1
100ul

Anti-EEF2 (phospho-T56) Antibody

Western blot (WB) analysis of p-EEF2 (T56) pAb at 1:500 dilution
Lane1:K562 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(10ug)
Lane3:The Liver tissue lysate of Rat(40ug)
Lane4:The Liver tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of p-EEF2 (T56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC, WB

$482
Cat # A00830T56-1
100ul

Anti-EEF2 (A50) Antibody

Western blot (WB) analysis of eEF2 (A50) polyclonal antibody at 1:500 dilution
Lane1:HepG2 cell lysate
Lane2:Mouse kidney tissue lysate
Lane3:Rat kidney tissue lysate

Immunohistochemistry (IHC) analyzes of EEF2 (A50) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IF, IHC, WB

$405
Cat # A00830
100ul

Anti-eEF2K (P360) Antibody

Western blot (WB) analysis of eEF2K (P360) polyclonal antibody at 1:1000 dillution
Lane1:Hela whole cell lysate(40μg)
Lane2:HCT-116 whole cell lysate(40μg)
Lane3:C6 whole cell lysate(60μg)

Immunohistochemistry (IHC) analyzes of eEF2K (P360) pAb in paraffin-embedded human breast carcinoma tissue at 1:100

Human, Mouse, Rat

IHC, WB

$405
Cat # A02277-2
100ul

Anti-Phospho-eEF2K (S366) Antibody

Western Blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HeLa cells treated with serum 10% 15

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody

Human, Rat

ELISA, IF, IHC, WB

$380
Cat # A02277S366-2
100ul

Anti-eEF2K Antibody

Western Blot analysis of HeLa cells using eEF2K Polyclonal Antibody diluted at 1:500

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2K Antibody

Human, Rat

ELISA, IF, IHC, WB

$399
Cat # A02277
100μl

eEF2 (Phospho-Thr56) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from NIH-3T3 cells treated with Serum 10% 30

Human, Mouse, Rat

ELISA

$540
Cat # EKC1985
1 kit, containing two 96-well plates and all necessary reagents

eEF2 Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from NIH-3T3 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1180
1 kit, containing one 96-well plate and all necessary reagents

eEF2K (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells treated with serum 10% 15

Human, Rat

ELISA

$540
Cat # EKC1986
1 kit, containing two 96-well plates and all necessary reagents

eEF2K Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells

Human, Rat

ELISA

$480
Cat # EKC1181
1 kit, containing one 96-well plate and all necessary reagents

FAM86A (EEF2KMT) (NM_201400) Human Over-expression Lysate

FAM86A (EEF2KMT) Human Over-expression Lysates NM_201400

Human

$628
Cat # LS196483
20 µg, 100 µg

EEF2 (NM_001961) Human Over-expression Lysate

Human

$960
Cat # LS001938
20 µg, 100 µg

Anti-Phospho-eEF2k (Ser366) Antibody

Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using (upper) or Beta-actin (lower)

Human

$299
Cat # M02277
50 µl

EEF2K (NM_013302) Human Over-expression Lysate

Human

$628
Cat # LS029904
20 µg, 100 µg

Anti-Phospho-eEF2K (S359) Antibody

Immunohistochemical analysis of paraffin-embedded human Colon cancer

Human, Mouse, Rat

ELISA, IF, IHC

$380
Cat # A02277S359
100ul

Anti-EEF2 Monoclonal Antibody

Western blot analysis of EEF2 expression in (1) A431 cell lysate; (2) NIH/3T3 cell lysate

Western blot analysis of EEF2 using anti-EEF2 antibody (M00830-1)

IHC analysis of EEF2 using anti-EEF2 antibody (M00830-1)

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M00830-1
100 μl

Human EEF2 Recombinant Protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

SDS-PAGE, Denatured

$250
Cat # PROTP13639
Starting from 20ug

EEF2K (NM_013302) Human Recombinant Protein

Coomassie blue staining of purified EEF2K protein

$1249
Cat # PROTO00418
20 µg

EEF2 Overview

Facts about Elongation factor 2.

EEF2 3D structure. Source: alphafold

Elongation factor 2 (EEF2)

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the recently formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA proceed to the P and E sites, respectively.

Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. .

Gene Information

Human
Gene Name:	EEF2
Uniprot:	P13639
Entrez:	1938

Family

Belongs to:

TRAFAC class translation factor GTPase superfamily

Alternative Names

eEF2; eEF-2; EF-2; EF2EEF-2; elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase

Molecular Weight

Mass (kDA):
95.338 kDA

Genomic Context


Human

Location:	19p13.3
Sequence:	19; NC_000019.10 (3976056..3985463, complement)

Subcellular Localizations

Cytoplasm. Nucleus. Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.

Diseases

Malignant Neoplasms
Hypertrophy
Diphtheria
Neoplasms
Hypoxia
Depressive Disorder
Ischemia
Diabetes Mellitus
Atrophy
Anoxia
Tissue Adhesions
Diabetes Mellitus, Non-insulin-dependent
Muscular Atrophy

Insulin Resistance
Tuberous Sclerosis
Sclerosis
Cardiac Hypertrophy
Carcinoma
Endoplasmic Reticulum Stress
Cholera

Interacting Proteins

Akt2
HSD17B10

Pathways

Translation
Cell Death
Regulation Of Translation
Cell Cycle
Localization
Pathogenesis
Translational Elongation
Cell Growth
Transport
Energy Homeostasis
Muscle Atrophy
Protein Phosphorylation
Induction Of Apoptosis

Mitosis
Autophagy
Muscle Hypertrophy
Hyperphosphorylation
Muscle Contraction
Virulence
Secretion

PTMs

Phosphorylation
Dephosphorylation
Ribosylation
Cleavage
Acetylation

Oxidation
Sumoylation
Biotinylation
Reduction
Methylation

Research Areas

Core ESC-Like Genes
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EEF2

References

PMID: 2610926 by Rapp G., et al. Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.

PMID: 1596361 by Hanes J., et al. Construction of a plasmid containing the complete coding region of human elongation factor 2.