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Product Info Summary
| SKU: | A12840 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-EGFL4 Antibody
SKU/Catalog Number
A12840
Size
100μl
Form
Liquid
Description
Boster Bio Anti-EGFL4 Antibody catalog # A12840. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGFL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12840)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MEGF8. AA range:1243-1292
Reactive Species
A12840 is reactive to MEGF8 in Human, Mouse, Rat
Calculated molecular weight
303.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12840 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using EGFL4 Polyclonal Antibody
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Western blot analysis of lysates from COLO cells, using MEGF8 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using MEGF8 antibody.
Specific Publications For Anti-EGFL4 Antibody (A12840)
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Customer Q&As
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4 Customer Q&As for Anti-EGFL4 Antibody
Question
Would anti-EGFL4 antibody A12840 work on bovine WB with tendon of biceps brachii?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
Our lab technicians have not validated anti-EGFL4 antibody A12840 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-EGFL4 antibody A12840 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine tendon of biceps brachii in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-07
Question
I am looking for to test anti-EGFL4 antibody A12840 on human plasma for research purposes, then I may be interested in using anti-EGFL4 antibody A12840 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The products we sell, including anti-EGFL4 antibody A12840, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-29
Question
Can you help my question with product A12840, anti-EGFL4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12840 anti-EGFL4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-EGFL4 antibody A12840 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
The anti-EGFL4 antibody (A12840) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-13
