Anti-EI2BG Antibody

Product Name

Anti-EI2BG Antibody

View all EIF2B3 Antibodies

SKU/Catalog Number

M08134

Size

50 µl

Description

Boster Bio Anti-EI2BG Antibody (Catalog # M08134). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-EI2BG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08134)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1778CT889.66.6.54

Isotype

IgG1,k

Immunogen

This EI2BG antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 110-452 amino acids from human EI2BG.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08134 is reactive to EIF2B3 in Human

Applications

M08134 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.24kDa

Background of EIF2B3

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000
FC: 1:25

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EI2BG with M08134 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

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All lanes : Anti-EI2BG Antibody at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: U-2OS whole cell lysate
Lane 4: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Overlay histogram showing U-2 OS cells stained with M08134 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08134, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For EIF2B3 (Source: Uniprot.org, NCBI)

Alternative Names

eIF-2B GDP-GTP exchange factor subunit gamma; EIF-2B; EIF2Bgamma; eukaryotic translation initiation factor 2B, subunit 3 (gamma, 58kD); eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa; translation initiation factor eIF-2B subunit gamma EIF2B3 EIF-2B, EIF2Bgamma eukaryotic translation initiation factor 2B subunit gamma translation initiation factor eIF-2B subunit gamma|eIF-2B GDP-GTP exchange factor subunit gamma|eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa

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