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Product Info Summary
| SKU: | A05656 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-EIF3H Antibody
SKU/Catalog Number
A05656
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EIF3H Antibody catalog # A05656. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF3H Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05656)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human EIF3H(NP_003747.1).
Reactive Species
A05656 is reactive to EIF3H in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
39.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05656 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of EIF3H pAb at 1:5000 dilution Lane1:EC9706 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:The Pancreas tissue lysate of Mouse(40ug) Lane5:The Thymus tissue lysate of Rat(40ug) Lane6:PC12 whole cell lysate(40ug) Lane7:RAW264.7 whole cell lysate(40ug)
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Immunohistochemical analysis of EIF3H staining in human colorectal cancer formalin fixed paraffin embedded tissue section.
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Immunofluorescence analysis of PC3 cells using EIF3H pAb at dilution of 1:50.
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Immunoprecipitation of Jurkat cell lysates using EIF3H pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 1 is 30% input. The western blot was probed using EIF3H pAb.
Specific Publications For Anti-EIF3H Antibody (A05656)
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