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Product Info Summary
| SKU: | A00846 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-eIF4B(Ab-422) Antibody
SKU/Catalog Number
A00846
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-eIF4B(Ab-422) Antibody (Catalog # A00846). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4B(Ab-422) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00846)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00846 is reactive to EIF4B in Human, Mouse, Rat
Applications
A00846 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
69.151kDa
Background of EIF4B
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from 3T3 and HL60 cells using eIF4B(Ab-422) antibody A00846.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B(Ab-422) antibody A00846(left) or the same antibody preincubated with blocking peptide(right).
Protein Target Info & Infographic
Gene/Protein Information For EIF4B (Source: Uniprot.org, NCBI)
Gene Name
EIF4B
Full Name
Eukaryotic translation initiation factor 4B
Weight
69.151kDa
Alternative Names
Eif4a2; eIF4B; EIF-4B; eukaryotic translation initiation factor 4B; PRO1843 EIF4B EIF-4B, PRO1843 eukaryotic translation initiation factor 4B eukaryotic translation initiation factor 4B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4B, check out the EIF4B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4B(Ab-422) Antibody (A00846)
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No publications found for A00846
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-eIF4B(Ab-422) Antibody
Question
Would anti-eIF4B(Ab-422) antibody A00846 work for IHC with female gonad?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of female gonad, EIF4B is highly expressed in female gonad. So, it is likely that anti-eIF4B(Ab-422) antibody A00846 will work for IHC with female gonad.
Boster Scientific Support
Answered: 2020-01-03
Question
Will anti-eIF4B(Ab-422) antibody A00846 work on goat IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Our lab technicians have not validated anti-eIF4B(Ab-422) antibody A00846 on goat. You can run a BLAST between goat and the immunogen sequence of anti-eIF4B(Ab-422) antibody A00846 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-09
Question
Can you help my question with product A00846, anti-eIF4B(Ab-422) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00846 anti-eIF4B(Ab-422) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-09
Question
I was wanting to use your anti-eIF4B(Ab-422) antibody for IHC for rat female gonad on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat female gonad identification?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can see on the product datasheet, A00846 anti-eIF4B(Ab-422) antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat female gonad in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-04
Question
Does A00846 anti-eIF4B(Ab-422) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
You can see on the product datasheet, A00846 anti-eIF4B(Ab-422) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
We are currently using anti-eIF4B(Ab-422) antibody A00846 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
A. Moore
Verified customer
Asked: 2018-05-04
Answer
The anti-eIF4B(Ab-422) antibody (A00846) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-04
