Product Info Summary
SKU: | A00846S422-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-eIF4B (phospho-S422) Antibody
SKU/Catalog Number
A00846S422-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eIF4B (phospho-S422) Antibody catalog # A00846S422-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4B (phospho-S422) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00846S422-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00846S422-1 is reactive to EIF4B in Human, Mouse, Rat
Applications
A00846S422-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
69.151kDa
Background of EIF4B
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of EIF4B using Anti-eIF4B (phospho-S422) Antibody (A00846S422-1).
Immunohistochemistry (IHC) analyzes of p-eIF4B (S422) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-eIF4B (phospho-S422) Antibody A00846S422-1
Western blot (WB) analysis of p-eIF4B (S422) pAb at 1:500 dilutionLane1:The Brain tissue lysate of Rat(40ug)
Lane2:K562 whole cell lysate(40ug)
Lane3:A375 whole cell lysate(40ug)
Lane4:HCT116 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EIF4B (Source: Uniprot.org, NCBI)
Gene Name
EIF4B
Full Name
Eukaryotic translation initiation factor 4B
Weight
69.151kDa
Alternative Names
Eif4a2; eIF4B; EIF-4B; eukaryotic translation initiation factor 4B; PRO1843 EIF4B EIF-4B, PRO1843 eukaryotic translation initiation factor 4B eukaryotic translation initiation factor 4B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4B, check out the EIF4B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4B (phospho-S422) Antibody (A00846S422-1)
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No publications found for A00846S422-1
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