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Product Info Summary
| SKU: | A03714-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Elf-4 Antibody
SKU/Catalog Number
A03714-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Elf-4 Antibody catalog # A03714-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Elf-4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03714-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ELF4. AA range:178-227
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03714-1 is reactive to ELF4 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
70.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03714-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of the lysates from RAW264.7cells using ELF4 antibody.
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Western Blot analysis of 22RV1 cells using Elf-4 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-Elf-4 Antibody (A03714-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Elf-4 Antibody
Question
Is this A03714-1 anti-Elf-4 antibody reactive to the isotypes of ELF4?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The immunogen of A03714-1 anti-Elf-4 antibody is Synthesized peptide derived from the Internal region of human Elf-4. at AA rangle: 150-230. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-12
Question
Is a blocking peptide available for product anti-Elf-4 antibody (A03714-1)?
B. Wu
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-Elf-4 antibody (A03714-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Elf-4 antibody A03714-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-28
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Elf-4 antibody A03714-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
Do you have a BSA free version of anti-Elf-4 antibody A03714-1 available?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Elf-4 antibody A03714-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-10
Question
We are currently using anti-Elf-4 antibody A03714-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
D. Miller
Verified customer
Asked: 2014-08-04
Answer
The anti-Elf-4 antibody (A03714-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-04
Question
Does anti-Elf-4 antibody A03714-1 work on goat WB with ewing sarcoma {eco:0000269?
B. Dhar
Verified customer
Asked: 2013-01-18
Answer
Our lab technicians have not validated anti-Elf-4 antibody A03714-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Elf-4 antibody A03714-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat ewing sarcoma {eco:0000269 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-18
