Product Info Summary
SKU: | A03091-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-ELMO1 Antibody
SKU/Catalog Number
A03091-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ELMO1 Antibody catalog # A03091-1. Tested in WB,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ELMO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03091-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Mlx
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03091-1 is reactive to ELMO1 in Human, Mouse, Rat
Applications
A03091-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
83.829kDa
Background of ELMO1
Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
McGuinness B.E., PLoS Biol. 3:433-449(2005).
Kitajima T.S., Curr. Biol. 15:353-359(2005).
Tang Z., Proc. Natl. Acad. Sci. U.S.A. 101:18012-18017(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ELMO1 using Anti-ELMO1 Antibody (A03091-1).
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-ELMO1 Antibody A03091-1
Western blot analysis of ELMO1 on mouse spleen tissue and Jurkat cell lysates using anti-ELMO1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For ELMO1 (Source: Uniprot.org, NCBI)
Gene Name
ELMO1
Full Name
Engulfment and cell motility protein 1
Weight
83.829kDa
Alternative Names
ced-12 homolog 1; CED12CED-12; ELMO-1; engulfment and cell motility 1 (ced-12 homolog, C. elegans); engulfment and cell motility 1; engulfment and cell motility protein 1; KIAA0281MGC126406; Protein ced-12 homolog ELMO1 CED-12, CED12, ELMO-1 engulfment and cell motility 1 engulfment and cell motility protein 1|ced-12 homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELMO1, check out the ELMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ELMO1 Antibody (A03091-1)
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No publications found for A03091-1
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