Anti-Lipocalin 2/LCN2 Biotinylated Antibody

Lipocalin-2/NGAL antibody

Boster Bio Anti-Lipocalin 2/LCN2 Biotinylated Antibody catalog # A00452-Biotin. Tested in ELISA applications. This antibody reacts with Mouse.

Product Info Summary

SKU: A00452-Biotin
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA

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Product Name

Anti-Lipocalin 2/LCN2 Biotinylated Antibody

View all Lipocalin-2/NGAL Antibodies

SKU/Catalog Number

A00452-Biotin

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lipocalin 2/LCN2 Biotinylated Antibody catalog # A00452-Biotin. Tested in ELISA applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lipocalin 2/LCN2 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00452-Biotin)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00452-Biotin is reactive to LCN2 in Mouse

Applications

A00452-Biotin is guaranteed for ELISA Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

22.588kDa

Background of Lipocalin-2/NGAL

Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For LCN2 (Source: Uniprot.org, NCBI)

Gene Name

LCN2

Full Name

Neutrophil gelatinase-associated lipocalin

Weight

22.588kDa

Superfamily

calycin superfamily

Alternative Names

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LCN2, check out the LCN2 Infographic

LCN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LCN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Lipocalin 2/LCN2 Biotinylated Antibody

Question

My question regarding product A00452-Biotin, anti-Lipocalin 2/LCN2 Biotinylated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00452-Biotin anti-Lipocalin 2/LCN2 Biotinylated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-07

Question

Will anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin work on bovine ELISA with neutrophil?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

Our lab technicians have not validated anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine neutrophil in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-24

Question

We want to test anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin on mouse bile for research purposes, then I may be interested in using anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Dhar

Verified customer

Asked: 2019-08-23

Answer

The products we sell, including anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-23

Question

Do you have a BSA free version of anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin available?

Verified Customer

Verified customer

Asked: 2019-04-03

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-03

Question

Does A00452-Biotin anti-Lipocalin 2/LCN2 Biotinylated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Zhao

Verified customer

Asked: 2018-09-19

Answer

You can see on the product datasheet, A00452-Biotin anti-Lipocalin 2/LCN2 Biotinylated antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-09-19

Question

I have attached the WB image, lot number and protocol we used for bile using anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-18

Question

Will anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin work for ELISA with bile?

T. Li

Verified customer

Asked: 2017-01-12

Answer

According to the expression profile of bile, LCN2 is highly expressed in bile. So, it is likely that anti-Lipocalin 2/LCN2 Biotinylated antibody A00452-Biotin will work for ELISA with bile.

Boster Scientific Support

Answered: 2017-01-12

Order DetailsPrice
A00452-Biotin

100μg

$515
A00452-Biotin-carrier-free

Carrier Free

$515

Free Secondary Antibody

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A00452-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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