Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody

Product Name

Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody

View all EEF1A1 Antibodies

SKU/Catalog Number

A02141-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody catalog # A02141-1. Tested in WB,ICC,IF,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02141-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human GIT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02141-1 is reactive to EEF1A1 in Human, Mouse, Rat

Applications

A02141-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50.141kDa

Background of EEF1A1

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.

Premont R.T., J. Biol. Chem. 275:22373-22380(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Premont R.T., Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

Click image to see more details

Figure 2. Immunocytochemistry staining of EEF1A1 using Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody (A02141-1).

ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

Click image to see more details

Figure 1. Western blotting validation for Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody A02141-1

Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain tissue
Lane 2: Mouse skeletal muscle
Lane 3: Mouse cerebellum
Lane 4: Rat skin
Lane 5: Daudi
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For EEF1A1 (Source: Uniprot.org, NCBI)

Alternative Names

CCS3; CCS-3; cervical cancer suppressor 3; CTCL tumor antigen; EE1A1; EEF-1; EEF1A; eEF1A-1; EF1AHNGC:16303; EF1a-like protein; EF-1-alpha-1; EF-Tu; elongation factor 1 alpha subunit; elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1; eukaryotic translation elongation factor 1 alpha 1-like 14; FLJ25721; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; LENG7; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; MGC102687; MGC131894; MGC16224; prostate tumor-induci EEF1A1 CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, LENG7, PTI1, eEF1A-1 eukaryotic translation elongation factor 1 alpha 1 elongation factor 1-alpha 1|CTCL tumor antigen|EF-1-alpha-1|EF1a-like protein|cervical cancer suppressor 3|elongation factor 1 alpha subunit|elongation factor Tu|epididymis secretory sperm binding protein|eukaryotic elongation factor 1 A-1|eukaryotic translation elongation factor 1 alpha 1-like 14|glucocorticoid receptor AF-1 specific elongation factor|leukocyte receptor cluster (LRC) member 7|leukocyte receptor cluster member 7|prostate tumor-inducing protein 1|translation elongation factor 1 alpha 1-like 14

For more info on EEF1A1, check out the EEF1A1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EEF1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the EEF1A1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis