Product Info Summary
SKU: | A02811-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-EphB1/2 Antibody
SKU/Catalog Number
A02811-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EphB1/2 Antibody catalog # A02811-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EphB1/2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02811-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human EphB1/2 around the non-phosphorylation site of Y594/604.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02811-2 is reactive to EPHB1 in Human, Mouse, Rat
Applications
A02811-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
109.885kDa
Background of EPHB1
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth.
Jiang-Hong Man, Nucleic Acids Res., Nov 2006; 34: 5552 - 5566.
Amir Sonnenblick, Mol. Cell. Biol., Dec 2004; 24: 10584 - 10592.
Jianyin Long, PNAS, Jan 2004; 101: 99.
Hyun Duk Jang, J. Biol. Chem., Jun 2004; 279: 24873 - 24880.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-EphB1/2 Antibody A02811-2
Western Blot (WB) analysis of specific cells using EphB1/2 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-EphB1/2 Antibody A02811-2
Western Blot (WB) analysis of HepG2 cells using EphB1/2 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EPHB1 (Source: Uniprot.org, NCBI)
Gene Name
EPHB1
Full Name
Ephrin type-B receptor 1
Weight
109.885kDa
Superfamily
protein kinase superfamily
Alternative Names
Cek6; EC 2.7.10; EC 2.7.10.1; EK6; Elk; EPH receptor B1; eph tyrosine kinase 2; EphB1; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2FLJ37986; Hek6; Net; NETHek6; soluble EPHB1 variant 1; Tyrosine-protein kinase receptor EPH-2 EPHB1 ELK, EPHT2, Hek6, NET EPH receptor B1 ephrin type-B receptor 1|EK6|EPH-like kinase 6|eph tyrosine kinase 2|neuronally-expressed EPH-related tyrosine kinase|soluble EPHB1 variant 1|tyrosine-protein kinase receptor EPH-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHB1, check out the EPHB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EphB1/2 Antibody (A02811-2)
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No publications found for A02811-2
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Customer Reviews
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