Anti-EPHB6/Eph Receptor B6 Antibody

EPHB6 antibody

Boster Bio Anti-EPHB6/Eph Receptor B6 Antibody (Catalog # A04270). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04270
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-EPHB6/Eph Receptor B6 Antibody

View all EPHB6 Antibodies

SKU/Catalog Number

A04270

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-EPHB6/Eph Receptor B6 Antibody (Catalog # A04270). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EPHB6/Eph Receptor B6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04270)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human EPHB6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04270 is reactive to EPHB6 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

Calculated molecular weight

110700 MW

Background of EPHB6

Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.

atsuoka H., Biochem. Biophys. Res. Commun. 235:487-492(1997).
Hillier L.W., Nature 424:157-164(2003).
Zhang Z., Protein Sci. 13:2819-2824(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A04270 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Positive Control

Validation Images & Assay Conditions

Gene/Protein Information For EPHB6 (Source: Uniprot.org, NCBI)

Gene Name

EPHB6

Full Name

Ephrin type-B receptor 6

Weight

110700 MW

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.10.1; EPH receptor B6; EphB6; ephrin type-B receptor 6; Hep; HEPTyrosine-protein kinase-defective receptor EPH-6; Mep; MGC129910; MGC129911 EPHB6 HEP EPH receptor B6 ephrin type-B receptor 6|human kinase-defective Eph-family receptor protein|tyrosine-protein kinase-defective receptor EPH-6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EPHB6, check out the EPHB6 Infographic

EPHB6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EPHB6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A04270

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-EPHB6/Eph Receptor B6 Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-EPHB6/Eph Receptor B6 Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

13 Customer Q&As for Anti-EPHB6/Eph Receptor B6 Antibody

Question

I see that the anti-EPHB6/Eph Receptor B6 antibody A04270 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-13

Question

Is there a BSA free version of anti-EPHB6/Eph Receptor B6 antibody A04270 available?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EPHB6/Eph Receptor B6 antibody A04270 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-10

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-EPHB6/Eph Receptor B6 antibody A04270. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-31

Question

Can you help my question with product A04270, anti-EPHB6/Eph Receptor B6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04270 anti-EPHB6/Eph Receptor B6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-05

Question

I have attached the WB image, lot number and protocol we used for brain using anti-EPHB6/Eph Receptor B6 antibody A04270. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-22

Question

Does anti-EPHB6/Eph Receptor B6 antibody A04270 work on monkey IF with brain?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

Our lab technicians have not tested anti-EPHB6/Eph Receptor B6 antibody A04270 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EPHB6/Eph Receptor B6 antibody A04270 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-14

Question

We are currently using anti-EPHB6/Eph Receptor B6 antibody A04270 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

The anti-EPHB6/Eph Receptor B6 antibody (A04270) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-10

Question

Does A04270 anti-EPHB6/Eph Receptor B6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

As indicated on the product datasheet, A04270 anti-EPHB6/Eph Receptor B6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-13

Question

Will anti-EPHB6/Eph Receptor B6 antibody A04270 work for WB with brain?

Verified Customer

Verified customer

Asked: 2018-06-29

Answer

According to the expression profile of brain, EPHB6 is highly expressed in brain. So, it is likely that anti-EPHB6/Eph Receptor B6 antibody A04270 will work for WB with brain.

Boster Scientific Support

Answered: 2018-06-29

Question

Is a blocking peptide available for product anti-EPHB6/Eph Receptor B6 antibody (A04270)?

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

We do provide the blocking peptide for product anti-EPHB6/Eph Receptor B6 antibody (A04270). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-22

Question

Is this A04270 anti-EPHB6/Eph Receptor B6 antibody reactive to the isotypes of EPHB6?

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

The immunogen of A04270 anti-EPHB6/Eph Receptor B6 antibody is Synthesized peptide derived from internal of human EPHB6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-03-07

Question

We are interested in to test anti-EPHB6/Eph Receptor B6 antibody A04270 on mouse brain for research purposes, then I may be interested in using anti-EPHB6/Eph Receptor B6 antibody A04270 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Kulkarni

Verified customer

Asked: 2016-11-04

Answer

The products we sell, including anti-EPHB6/Eph Receptor B6 antibody A04270, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-11-04

Question

I was wanting to use your anti-EPHB6/Eph Receptor B6 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?

B. Thomas

Verified customer

Asked: 2016-10-06

Answer

It shows on the product datasheet, A04270 anti-EPHB6/Eph Receptor B6 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-10-06

Order DetailsPrice
A04270-100uL

100uL

$360

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A04270
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.