Click image to see more details
Product Info Summary
| SKU: | A04270 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EPHB6/Eph Receptor B6 Antibody
SKU/Catalog Number
A04270
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EPHB6/Eph Receptor B6 Antibody (Catalog # A04270). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPHB6/Eph Receptor B6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04270)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human EPHB6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04270 is reactive to EPHB6 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
110700 MW
Background of EPHB6
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
atsuoka H., Biochem. Biophys. Res. Commun. 235:487-492(1997).
Hillier L.W., Nature 424:157-164(2003).
Zhang Z., Protein Sci. 13:2819-2824(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04270 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHB6 antibody A04270.
Click image to see more details
Western blot analysis of extracts from HeLa cells, 293 cells and COLO cells, using EPHB6 antibody A04270.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using EPHB6 antibody A04270.
Protein Target Info & Infographic
Gene/Protein Information For EPHB6 (Source: Uniprot.org, NCBI)
Gene Name
EPHB6
Full Name
Ephrin type-B receptor 6
Weight
110700 MW
Superfamily
protein kinase superfamily
Alternative Names
Ephrin type-B receptor 6;HEP;Tyrosine-protein kinase-defective receptor EPH-6;EPHB6; EPHB6 HEP EPH receptor B6 ephrin type-B receptor 6|human kinase-defective Eph-family receptor protein|tyrosine-protein kinase-defective receptor EPH-6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHB6, check out the EPHB6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHB6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPHB6/Eph Receptor B6 Antibody (A04270)
Hello CJ!
No publications found for A04270
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EPHB6/Eph Receptor B6 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EPHB6/Eph Receptor B6 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-EPHB6/Eph Receptor B6 Antibody
Question
I see that the anti-EPHB6/Eph Receptor B6 antibody A04270 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-13
Question
Is there a BSA free version of anti-EPHB6/Eph Receptor B6 antibody A04270 available?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EPHB6/Eph Receptor B6 antibody A04270 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-EPHB6/Eph Receptor B6 antibody A04270. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
Can you help my question with product A04270, anti-EPHB6/Eph Receptor B6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04270 anti-EPHB6/Eph Receptor B6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-05
Question
I have attached the WB image, lot number and protocol we used for brain using anti-EPHB6/Eph Receptor B6 antibody A04270. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
Does anti-EPHB6/Eph Receptor B6 antibody A04270 work on monkey IF with brain?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Our lab technicians have not tested anti-EPHB6/Eph Receptor B6 antibody A04270 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EPHB6/Eph Receptor B6 antibody A04270 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
We are currently using anti-EPHB6/Eph Receptor B6 antibody A04270 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The anti-EPHB6/Eph Receptor B6 antibody (A04270) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-10
Question
Does A04270 anti-EPHB6/Eph Receptor B6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
As indicated on the product datasheet, A04270 anti-EPHB6/Eph Receptor B6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-13
Question
Will anti-EPHB6/Eph Receptor B6 antibody A04270 work for WB with brain?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
According to the expression profile of brain, EPHB6 is highly expressed in brain. So, it is likely that anti-EPHB6/Eph Receptor B6 antibody A04270 will work for WB with brain.
Boster Scientific Support
Answered: 2018-06-29
Question
Is a blocking peptide available for product anti-EPHB6/Eph Receptor B6 antibody (A04270)?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
We do provide the blocking peptide for product anti-EPHB6/Eph Receptor B6 antibody (A04270). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-22
Question
Is this A04270 anti-EPHB6/Eph Receptor B6 antibody reactive to the isotypes of EPHB6?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
The immunogen of A04270 anti-EPHB6/Eph Receptor B6 antibody is Synthesized peptide derived from internal of human EPHB6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-07
Question
We are interested in to test anti-EPHB6/Eph Receptor B6 antibody A04270 on mouse brain for research purposes, then I may be interested in using anti-EPHB6/Eph Receptor B6 antibody A04270 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Kulkarni
Verified customer
Asked: 2016-11-04
Answer
The products we sell, including anti-EPHB6/Eph Receptor B6 antibody A04270, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-04
Question
I was wanting to use your anti-EPHB6/Eph Receptor B6 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
B. Thomas
Verified customer
Asked: 2016-10-06
Answer
It shows on the product datasheet, A04270 anti-EPHB6/Eph Receptor B6 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-06
