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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Rat MCP-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat MCP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ccl2 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat MCP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0902)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q24-N148
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0902 is reactive to Ccl2 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK0902 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ccl2
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3
|EK0902-CAP||Anti-Rat Ccl2 Pre-coated 96-well strip microplate||1|
|EK0902-ST||Rat Ccl2 Standard||2 vials, 10 ng/tube|
|EK0902-DA||Rat Ccl2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat MCP-1/CCL2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat MCP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Ccl2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ccl2 (Source: Uniprot.Org, NCBI)
C-C motif chemokine 2
intercrine beta (chemokine CC) family
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF Ccl2|MCP-1, Scya2, Sigje|C-C motif chemokine ligand 2|C-C motif chemokine 2|chemokine (C-C motif) ligand 2|immediate-early serum-responsive JE protein|immediate-early serum-responsive protein JE|monocyte chemoattractant protein 1|monocyte chemotactic protein 1|small inducible cytokine A2|small inducible gene JE*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ccl2, check out the Ccl2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ccl2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0902 has been cited in 43 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comparison of biomarkers in rat renal ischemia-reperfusion injury
Tanshinone IIA attenuates cerebral aneurysm formation by inhibiting the NF‑κB‑mediated inflammatory response
Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE−/− Mice through the ROS/MAPK/NF-κB Pathway
The Potassium SK Channel Activator NS309 Protects Against Experimental Traumatic Brain Injury Through Anti-Inflammatory and Immunomodulatory Mechanisms
Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer
Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis
IL-17A promotes the neuroinflammation and cognitive function in sevoflurane anesthetized aged rats via activation of NF-κB signaling pathway
A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats
Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells
Crocetin attenuating Urinary tract Infection and adherence of uropathogenic E. coli in NRK-52E cells via an inflammatory pathway
Anti-atherosclerosis of oligomeric proanthocyanidins from Rhodiola rosea on rat model via hypolipemic, antioxidant, anti-inflammatory activities together with regulation of endothelial function
Fish-oil emulsion (omega-3 polyunsaturated fatty acids) attenuates acute lung injury induced by intestinal ischemia–reperfusion through Adenosine 5′-monophosphate-activated protein kinase–sirtuin1 pathway
Flaxseed oil alleviates dextran sulphate sodium-induced ulcerative colitis in rats
Effect of acetazolamide on cytokines in rats exposed to high altitude
Sevoflurane reduces inflammatory factor expression, increases viability and inhibits apoptosis of lung cells in acute lung injury by microRNA-34a-3p upregulation and STAT1 downregulation
Wedelolactone alleviates doxorubicin-induced inflammation and oxidative stress damage of podocytes by IκK/IκB/NF-κB pathway
Intervention effect and mechanism of curcumin in chronic urinary tract infection in rats
Pulmonary and pleural toxicity of potassium octatitanate fibers, rutile titanium dioxide nanoparticles, and MWCNT-7 in male Fischer 344 rats
Ligustrazine suppresses neuron apoptosis via the Bax/Bcl‐2 and caspase‐3 pathway in PC12 cells and in rats with vascular dementia
The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats
Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis
Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway
Zhang L, Xu C, Hu W, Wu P, Qin C, Zhang J. Int J Mol Med. 2018 Mar;41(3):1293-1304. doi: 10.3892/ijmm.2017.3327. Epub 2017 Dec 18. Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway
Liu X, Zhang J, Han W, Wang Y, Liu Y, Zhang Y, Zhou D, Xiang L. Mol Med Rep. 2017 Jul;16(1):192-200. doi: 10.3892/mmr.2017.6553. Epub 2017 May 9. Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats
Wei N, Zhang C, He H, Wang T, Liu Z, Liu G, Sun Z, Zhou Z, Bai C, Yuan D. J Pharm Pharmacol. 2014 Nov;66(11):1641-51. Doi: 10.1111/Jphp.12291. Epub 2014 Aug 24. Protective Effect Of Saponins Extract From Panax Japonicus On Myocardial Infarction: I...
Wang Zm, Gao W, Wang H, Zhao D, Nie Zl, Shi Jq, Zhao S, Lu X, Wang Ls, Yang Zj. Cell Physiol Biochem. 2014;33(5):1349-58. Doi: 10.1159/000358702. Epub 2014 May 5. Green Tea Polyphenol Epigallocatechin-3-Gallate Inhibits Tnf-??-Induced Production O...
Xu Y, Kong X, Zhou H, Zhang X, Liu J, Yan J, Xie H, Xie Y. Thromb Res. 2014 Aug;134(2):384-92. Doi: 10.1016/J.Thromres.2014.05.017. Epub 2014 May 20. Oxldl/??2Gpi/Anti-??2Gpi Complex Induced Macrophage Differentiation To Foam Cell Involving Tlr4/N...
Meneses Gc, Lib??rio Ab, De Daher Ef, Da Silva Gb Jr, Da Costa Mf, Pontes Ma, Martins Am. Bmc Infect Dis. 2014 Aug 20;14:451. Doi: 10.1186/1471-2334-14-451. Urinary Monocyte Chemotactic Protein-1 (Mcp-1) In Leprosy Patients: Increased Risk For Kid...
Jiang Jx, Zhang Sj, Xiong Yk, Jia Yl, Sun Yh, Lin Xx, Shen Hj, Xie Qm, Yan Xf. Plos One. 2015 Jun 2;10(6):E0128278. Doi: 10.1371/Journal.Pone.0128278. Ecollection 2015. Eets Attenuate Ox-Ldl-Induced Ltb4 Production And Activity By Inhibiting P38 M...
Aizman I, Vinodkumar D, Mcgrogan M, Bates D. Stem Cells Dev. 2015 Jul 15;24(14):1623-34. Doi: 10.1089/Scd.2015.0083. Epub 2015 May 20. Cell Injury-Induced Release Of Fibroblast Growth Factor 2: Relevance To Intracerebral Mesenchymal Stromal Cell T...
Tu J, Yang L, Zhou X, Qi K, Wang J, Kouadir M, Xu L, Yin X, Zhao D. J Mol Neurosci. 2014 Jan;52(1):107-16. Doi: 10.1007/S12031-013-0140-3. Epub 2013 Nov 13. Prp106-126 And A?? 1-42 Peptides Induce Bv-2 Microglia Chemotaxis And Proliferation.
Tu J, Chen B, Yang L, Qi K, Lu J, Zhao D. J Mol Neurosci. 2015 Jun;56(2):509-18. Doi: 10.1007/S12031-015-0553-2. Epub 2015 Apr 14. Amyloid-?? Activates Microglia And Regulates Protein Expression In A Manner Similar To Prions.
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.
Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...
Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...
Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...
Jiang Jx, Zhang Sj, Liu Yn, Lin Xx, Sun Yh, Shen Hj, Yan Xf, Xie Qm. Eur J Pharmacol. 2014 Mar 15;727:43-51. Doi: 10.1016/J.Ejphar.2014.01.045. Epub 2014 Jan 31. Eets Alleviate Ox-Ldl-Induced Inflammation By Inhibiting Lox-1 Receptor Expression In...
Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...
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