Product Info Summary
SKU: | A01770-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ERCC5/XPG (E160) Antibody
SKU/Catalog Number
A01770-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ERCC5/XPG (E160) Antibody catalog # A01770-1. Tested in WB,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC5/XPG (E160) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01770-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 130-180 of Human ERCC5/XPG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01770-1 is reactive to ERCC5 in Human
Applications
A01770-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
133.108kDa
Background of XPG
Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
Scherly D., Nature 363:182-185(1993).
Shiomi T., Mutat. Res. 314:167-175(1994).
Macinnes M.A., Mol. Cell. Biol. 13:6393-6402(1993).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot (WB) analysis of ERCC5/XPG (E160) pAb at 1:1000 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(20ug) Lane3:HCT116 whole cell lysate(20ug)
Click image to see more details
Immunohistochemistry (IHC) analyzes of ERCC5/XPG (E160) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.
Protein Target Info & Infographic
Gene/Protein Information For ERCC5 (Source: Uniprot.org, NCBI)
Gene Name
ERCC5
Full Name
DNA repair protein complementing XP-G cells
Weight
133.108kDa
Superfamily
XPG/RAD2 endonuclease family
Alternative Names
COFS3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; EC 3.1; ERCM2; excision repair cross-complementing rodent repair deficiency, complementationgroup 5; excision repair protein; xeroderma pigmentosum complementation group G protein; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum, complementation group G; XPGC; XPG-complementing protein; XPGUVDR ERCC5 COFS3-201, ERCM2, UVDR, XPG, XPGC, ERCC5 ERCC excision repair 5, endonuclease DNA repair protein complementing XP-G cells|DNA excision repair protein ERCC-5|XPG-complementing protein|excision repair cross-complementation group 5|excision repair cross-complementing rodent repair deficiency, complementation group 5|xeroderma pigmentosum, complementation group G
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC5, check out the ERCC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERCC5/XPG (E160) Antibody (A01770-1)
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No publications found for A01770-1
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