Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 1(MAPK1) detection. Tested with WB in Human;Mouse;Rat. Cited in 8 publication(s).
Anti-ERK2/MAPK1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-ERK2/MAPK1 Antibody
See all ERK2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for ERK2/MAPK1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. ERK2/MAPK1 information: Molecular Weight: 41390 MW; Subcellular Localization: Cytoplasm, cytoskeleton, spindle . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Associated with the spindle during prometaphase and metaphase (By similarity). PEA15- binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.|
|Cite This Product||Anti-ERK2/MAPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1310)|
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|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ERK2(348-360aa FEETARFQPGYRS), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MAPK1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5594|
|Species Of This Entry||Human|
|Protein Name||Mitogen-activated protein kinase 1|
|Superfamily||protein kinase superfamily|
|Alternative Names||ERK2|EC 2.7.11; EC 22.214.171.124; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Ubiquitination; Methylation; Reduction; Acetylation; Prenylation; Nitrosylation; Biotinylation; Nitration; Glycosylation; Oxidation; Myristoylation|
|Subcellular Localization||Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Membrane, caveola. Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization.|
|Gene Location||22q11.22, on Chromasome 22, gene sequence: NC_000022.11 (21759657..21867680, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MAPK1 (Source: Uniprot.org, NCBI)
|Protein Function||Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).|
|Interacting Proteins||ARRB2; BLVRA; COPS6; DUSP1; Dusp2; DUSP4; DUSP5; ETS1; MAP2K1; MAPK3; MKNK1; PEA15; PPM1A; PTPN7; PTPRJ; PTPRR; RPS6KA1; RPS6KA2; RPS6KA3; TRAPPC4|
|Research Areas||Angiogenesis; Apoptosis; Breast Cancer; Cancer; Diabetes Research; Hypoxia; Phospho-Specific; Protein Kinase; Signal Transduction; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Inflammation; Cell Invasion; Tissue Adhesions; Malignant Neoplasm Of Breast; Mammary Neoplasms; Neoplasm Metastasis; Tumor Angiogenesis; Cell Transformation, Neoplastic; Neoplasm Invasiveness; Malignant Paraganglionic Neoplasm; Leukemia; Adenocarcinoma; Malignant Neoplasm Of Prostate; Malignant Neoplasm Of Lung; Hypoxia; Diabetes Mellitus|
|Related Pathways||Cell Proliferation; Cell Cycle; Cell Death; Cell Growth; Secretion; Pathogenesis; Angiogenesis; Cell Migration; Localization; Cell Adhesion; Cell Differentiation; Translation; Transport; Rna Interference; Wound Healing; Cell Cycle Arrest; Chemotaxis; Immune Response; Drug Resistance; Senescence|
|Background||Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98% identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.|
|Scientific References||PMID: 1540184 by Owaki H., et al. Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs.|
PMID: 1319925 by Gonzalez F.A., et al. Heterogeneous expression of four MAP kinase isoforms in human tissues.
Our Boster Quality Guarantee for Anti-ERK2/MAPK1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Placenta Tissue Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: COLO320 Cell Lysate
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Total number of citations: 8
|Li Xl, Chen Xq, Zhang Mn, Chen N, Nie L, Xu M, Gong J, Shen Pf, Su Zz, Weng X, Tan Jy, Zhao T, Zeng H, Zhou Q. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3871-81. Ecollection 2015. Sox9 Was Involved In Tkis Resistance In Renal Cell Carcinoma Via Raf/M...||PubMed ID 26097571|
|Sang J, Chen Y, Tao Y. Mol Med Rep. 2011 Nov-Dec;4(6):1163-7. Doi: 10.3892/Mmr.2011.535. Epub 2011 Jul 15. Nitric Oxide Inhibits Gastric Cancer Cell Growth Through The Modulation Of The Akt Pathway.||PubMed ID 21769431|
|Ma Hr, Wang J, Chen Yf, Chen H, Wang Ws, Aisa Ha. Int J Mol Med. 2014 Jun;33(6):1627-34. Doi: 10.3892/Ijmm.2014.1722. Epub 2014 Apr 3. Icariin And Icaritin Stimulate The Proliferation Of Skbr3 Cells Through The Gper1-Mediated Modulation Of The Egf...||PubMed ID 24718680|
|eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation||PubMed ID 28193911|
|Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway||PubMed ID 26517513|
|The effects of demecolcine, alone or in combination with sucrose on bovine oocyte protrusion rate, MAPK1 protein level and c-mos gene expression level||PubMed ID 28358237|
|High levels of glucose promote the activation of hepatic stellate cells via the p38-mitogen-activated protein kinase signal pathway||PubMed ID 27706632|
|Glucagon-like peptide-1 receptor agonists inhibit hepatic stellate cell activation by blocking the p38 MAPK signaling pathway||PubMed ID 26782560|
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