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- Table of Contents
MAPK1 · Western blot design guide
MAPK1 Western Blot Planning Guide
Plan a MAPK1 Western blot around the catalog-observed 41.4 kDa band, image-backed PA1049 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
MAPK1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 41.4 kDa observed | |
| Observed band | 41.4 kDa observed | |
| Gel | 12-15% | |
| Positive control | Caudate | |
| Negative control | Use knockout/knockdown or orthogonal negative |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 41.4 kDa |
| ⓘ | Localization | Cytoplasm, cytoskeleton, spindle / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Rat / Mouse / Human |
Section 1
Real Curated MAPK1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adipose tissue |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA1049 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected MAPK1 Western Blot Band Size?
Use the product-observed 41.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 41.4 kDa | Matches the authoritative product WB observation. |
| 41.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected MAPK1 appearancePlan around 41.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 41.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 41.4 kDa from UniProt P28482; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA1049. |
| Situation | Likely cause | Next action |
|---|---|---|
| 41.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing MAPK1 band
PA1049 · MAPK1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MAPK1 Western blot
🧪Use Caudate as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Caudate (High)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for MAPK1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adipose tissue | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Epididymis | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Liver | Reported tissue cells | Low | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|
Section 3
Advanced MAPK1 Western Blot Tips
Deeper troubleshooting and optimisation questions for MAPK1, answered from its protein features.
Which band should guide the blot?
Use 41.4 kDa, the observation attached to the authoritative PA1049 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 41.4 kDa expectation.
Which positive control should I start with?
Start with Caudate, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use a target knockdown/knockout control when no HPA Not detected tissue is available.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for MAPK1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA1049 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
MAPK1 Western Blot Reagents
Rat/Mouse/Human-reactive MAPK1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-MAPK1/3 Antibody Picoband®
Cat # PA1049
Real WB data
Anti-ERK2/MAPK1 Antibody Picoband®
Cat # PA1310-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P28482
- Human Protein Atlas — MAPK1
- PA1049 product record
- PMC6270553 — Proteomic and functional analyses reveal MAPK1 regulates milk protein synthesis (Molecules (Basel, Switzerland), 2012)
- PMC10758092 — MAPK1 Mediates MAM Disruption and Mitochondrial Dysfunction in Diabetic Kidney Disease via the PACS-2-Dependent Mechanism (International journal of biological sciences, 2024)