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Product Info Summary
| SKU: | A05659-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-ERN2 Antibody
SKU/Catalog Number
A05659-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ERN2 Antibody (Catalog# A05659-1). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05659-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ERN2. Immunogen sequence location: 841-890
Reactive Species
A05659-1 is reactive to ERN2 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
102.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05659-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ERN2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using ERN2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ERN2 Antibody (A05659-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ERN2 Antibody
Question
Can A05659-1 work for Western Bot?
Verified customer
Asked: 2021-02-21
Answer
The Anti-ERN2 Antibody (A05659-1) is not recommended for WB.
Boster Scientific Support
Answered: 2021-02-22
Question
I have a question about product A05659-1, anti-ERN2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05659-1 anti-ERN2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-ERN2 antibody A05659-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-ERN2 antibody (A05659-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
Question
See attached the WB image, lot number and protocol we used for nasal cavity epithelium using anti-ERN2 antibody A05659-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Will anti-ERN2 antibody A05659-1 work on primate IF with embl:bab21297.1}?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
Our lab technicians have not tested anti-ERN2 antibody A05659-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ERN2 antibody A05659-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate embl:bab21297.1} in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-18
Question
Is a blocking peptide available for product anti-ERN2 antibody (A05659-1)?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
We do provide the blocking peptide for product anti-ERN2 antibody (A05659-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-05-31
Question
I was wanting to use your anti-ERN2 antibody for IF for mouse nasal cavity epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse nasal cavity epithelium identification?
M. Roberts
Verified customer
Asked: 2014-11-04
Answer
As indicated on the product datasheet, A05659-1 anti-ERN2 antibody has been tested for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse nasal cavity epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-04
