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Product Info Summary
| SKU: | PA1679 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ERp57/PDIA3 Antibody
View all ERp57/PDIA3 Antibodies
SKU/Catalog Number
PA1679
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ERp57/PDIA3 Antibody catalog # PA1679. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERp57/PDIA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1679)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ERp57, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1679 is reactive to PDIA3 in Human, Mouse, Rat
Applications
PA1679 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
56.782kDa
Background of ERp57/PDIA3
PDIA3 (Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme. It is mapped on 15q15.3. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Anti-ERp57 antibody, PA1679, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
Click image to see more details
Anti-ERp57 antibody, PA1679, IHC(P)
IHC(P): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For PDIA3 (Source: Uniprot.org, NCBI)
Gene Name
PDIA3
Full Name
Protein disulfide-isomerase A3
Weight
56.782kDa
Superfamily
protein disulfide isomerase family
Alternative Names
Disulfide isomerase ER-60; EC 5.3.4.1; endoplasmic reticulum P58; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER protein 60; ER60; ERp57; ERp5758 kDa glucose-regulated protein; ERp60; ERp6058 kDa microsomal protein; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; GRP58ER protein 57; HsT17083; P58; PDIA3; phospholipase C-alpha; PI-PLC; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; protein disulfide-isomerase A3 PDIA3 ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC protein disulfide isomerase family A member 3 protein disulfide-isomerase A3|58 kDa glucose-regulated protein|58 kDa microsomal protein|ER protein 57|ER protein 60|disulfide isomerase ER-60|endoplasmic reticulum P58|endoplasmic reticulum resident protein 57|endoplasmic reticulum resident protein 60|epididymis secretory protein Li 269|epididymis secretory sperm binding protein Li 93n|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDIA3, check out the PDIA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDIA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERp57/PDIA3 Antibody (PA1679)
Hello CJ!
PA1679 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shen L, Chen C, Yang A, Chen Y, Liu Q, Ni J. J Proteomics. 2015 Jun 18;123:101-13. Doi: 10.1016/J.Jprot.2015.04.005. Epub 2015 Apr 15. Redox Proteomics Identification Of Specifically Carbonylated Proteins In The Hippocampi Of Triple Transgenic Alz...
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