Product Info Summary
SKU: | A00536-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Exonuclease 1/EXO1 Antibody Picoband™
View all Exonuclease 1 Antibodies
SKU/Catalog Number
A00536-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Exonuclease 1/EXO1 Antibody Picoband™ catalog # A00536-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Exonuclease 1/EXO1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00536-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Exonuclease 1/EXO1 recombinant protein (Position: Q25-Q846).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00536-3 is reactive to EXO1 in Human
Applications
A00536-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
94.103kDa
Background of Exonuclease 1
Exonuclease 1is anenzymethat in humans is encoded by theEXO1gene. It is mapped to 1q43. This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exonuclease 1/EXO1 antigen affinity purified polyclonal antibody (Catalog # A00536-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Exonuclease 1/EXO1 at approximately 120 kDa. The expected band size for Exonuclease 1/EXO1 is at 94 kDa.
Click image to see more details
Figure 2. IF analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-3).
Exonuclease 1/EXO1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Exonuclease 1/EXO1 Antibody (A00536-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For EXO1 (Source: Uniprot.org, NCBI)
Gene Name
EXO1
Full Name
Exonuclease 1
Weight
94.103kDa
Superfamily
XPG/RAD2 endonuclease family
Alternative Names
EXOI; exonuclease 1; Exonuclease I; HEX1rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1; hExo1; hExoIEC 3.1 EXO1 HEX1, hExoI exonuclease 1 exonuclease 1|rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EXO1, check out the EXO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Exonuclease 1/EXO1 Antibody Picoband™ (A00536-3)
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