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Product Info Summary
| SKU: | A00050-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-EZH2 Antibody
SKU/Catalog Number
A00050-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-EZH2 Antibody (Catalog # A00050-3). Tested in WB, IHC-P, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EZH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00050-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EZH2 antibody is generated from rabbits immunized with a recombinant fragment (N-term) protein from human EZH2.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00050-3 is reactive to EZH2 in Human, Mouse
Calculated molecular weight
85.4 kDa
Background of EZH2
EZH2 is a protein that transfers sulfate to the C-4 hydroxyl of beta-1,4-linked GalNAc flanked by GlcUA residues in chondroitin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00050-3 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
IHC-P: 1:10-1:50
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling EZH2 with A00050-3 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).
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Western blot analysis of lysates from Jurkat, T47D cell line (from left to right), using EZH2 Antibody. A00050-3 was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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All lanes : Anti-EZH2 Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: Neuro-2a whole cell lysate
Lane 5: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. A00050-3 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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All lanes : Anti-EZH2 Antibody at 1:2000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: MDA-MB-231 whole cell lysates
Lane 3: MDA-MB-468 whole cell lysates
Lane 4: T47D whole cell lysates
Lane 5: mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-EZH2 Antibody at 1:2000 dilution
Lane 1: mouse testis lysates
Lane 2: MDA-MB-231 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Anti-EZH2 Antibodyat 1:500 dilution + MDA-MB-468 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-EZH2 Antibody (A00050-3)
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