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Product Info Summary
| SKU: | A00050 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-EZH2 Antibody
SKU/Catalog Number
A00050
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EZH2 Antibody (Catalog # A00050). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
EZH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-EZH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00050)
Host
Rabbit
Contents
EZH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2. The immunogen is located within amino acids 190 - 240 of EZH2.
Cross-reactivity
Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.
Reactive Species
A00050 is reactive to EZH2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
85.4 kDa
Background of EZH2
EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00050 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EZH2 antibody can be used for detection of EZH2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 μg/mL.
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Immunocytochemistry of EZH2 in 293 cells with EZH2 antibody at 10 μg/mL.
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Immunofluorescence of EZH2 in 293 cells with EZH2 antibody at 20 μg/mL.
Specific Publications For Anti-EZH2 Antibody (A00050)
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