Anti-F-BAR domain only protein 2 FCHO2 Antibody

Product Name

Anti-F-BAR domain only protein 2 FCHO2 Antibody

View all FCHO2 Antibodies

SKU/Catalog Number

A08075

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-F-BAR domain only protein 2 FCHO2 Antibody (Catalog # A08075). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FCHO2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-F-BAR domain only protein 2 FCHO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08075)

Host

Rabbit

Contents

FCHO2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FCHO2 antibody was raised against a 15 amino acid synthetic peptide near the center of human FCHO2. The immunogen is located within amino acids 340 - 390 of FCHO2.

Cross-reactivity

FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist; this antibody will detect only the two longest isoforms.

Reactive Species

A08075 is reactive to FCHO2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

88.9 kDa

Background of FCHO2

FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08075 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FCHO2

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