Anti-FAAH1/FAAH Antibody Picoband™

Product Name

Anti-FAAH1/FAAH Antibody Picoband™

View all FAAH Antibodies

SKU/Catalog Number

A00801-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FAAH1/FAAH Antibody Picoband™ catalog # A00801-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-FAAH1/FAAH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00801-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FAAH1/FAAH recombinant protein (Position: R34-S579).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00801-2 is reactive to FAAH in Human, Mouse, Rat

Applications

A00801-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

63.066kDa

Background of FAAH

Fatty acid amide hydrolase or FAAH (EC 3.5.1.99, oleamide hydrolase, anandamide amidohydrolase) is a member of the serine hydrolase family of enzymes. This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FAAH1/FAAH using anti-FAAH1/FAAH antibody (A00801-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAAH1/FAAH antigen affinity purified polyclonal antibody (Catalog # A00801-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAAH1/FAAH at approximately 63 kDa. The expected band size for FAAH1/FAAH is at 63 kDa.

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Figure 2. IF analysis of FAAH1/FAAH using anti-FAAH1/FAAH antibody (A00801-2).
FAAH1/FAAH was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FAAH1/FAAH Antibody (A00801-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-FAAH1/FAAH antibody (A00801-2).
Overlay histogram showing THP-1 cells stained with A00801-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAAH1/FAAH Antibody (A00801-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For FAAH (Source: Uniprot.org, NCBI)

Alternative Names

Anandamide amidohydrolase 1; EC 3.5.1; EC 3.5.1.99; EC 3.5.1.n2; FAAH1; FAAH-1; fatty acid amide hydrolase; fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase 1 FAAH FAAH-1, PSAB fatty acid amide hydrolase fatty-acid amide hydrolase 1|anandamide amidohydrolase 1|fatty acid ester hydrolase|oleamide hydrolase 1

For more info on FAAH, check out the FAAH Infographic

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In this infographic, you will see the following information for FAAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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