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Product Info Summary
| SKU: | A01220-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-FADS2 Antibody (Center)
SKU/Catalog Number
A01220-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-FADS2 Antibody (Center) (Catalog # A01220-1). Tested in IHC-P, WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FADS2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01220-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-122 amino acids of human FADS2.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01220-1 is reactive to FADS2 in Human
Calculated molecular weight
52.3 kDa
Background of FADS2
The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01220-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:10-1:50
Validation Images & Assay Conditions
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FADS2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H.brain section using FADS2 Antibody (Center). A01220-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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FADS2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-FADS2 Antibody (Center) (A01220-1)
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