Anti-Phospho-FAM129B (Ser679/683) Antibody

Product Name

Anti-Phospho-FAM129B (Ser679/683) Antibody

View all NIBAN2 Antibodies

SKU/Catalog Number

P09391

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-FAM129B (Ser679/683) Antibody (Catalog # P09391). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-FAM129B (Ser679/683) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P09391)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser679/683 of human FAM129B, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P09391 is reactive to NIBAN2 in Human, Mouse

Applications

P09391 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

84.138kDa

Background of NIBAN2

FAM129B, also known as Niban-like protein 1, belongs to a poorly characterized protein family with unknown category and function. Increased expression of the Niban gene has been observed in renal carcinomas (Adachi et al., 2004; Sun et al., 2007). Suppression of FAM129B expression in HeLa cells has been seen to promote apoptosis, suggesting that it can modulate cell death signaling, and may be involved in the ER stress response (Sun et al., 2007). FAM129B is also up-regulated in various types of thyroid tumors and Hashimoto’s thyroiditis (Matsumoto et al., 2006). It has been suggested that the MAP kinase dependent phosphorylation of FAM129B is important in controlling melanoma cells, as inhibition of B/Raf/MKK/ERK in melanoma cells represses invasion (Old et al., 2009). It is believed that phosphorylated FAM129B not only derepresses invasion, but also regulates events that promote invasion (Old et al., 2009).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser^679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For NIBAN2 (Source: Uniprot.org, NCBI)

Alternative Names

Protein Niban 2 NIBAN2 C9orf88, FAM129B, MEG-3, MINERVA, OC58, bA356B19.6 niban apoptosis regulator 2 protein Niban 2|family with sequence similarity 129 member B|melanoma invasion by ERK|niban-like protein 1

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