Anti-FANCA/Faca Antibody

Product Name

Anti-FANCA/Faca Antibody

View all FACA/FANCA Antibodies

SKU/Catalog Number

A03662

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-FANCA/Faca Antibody (Catalog # A03662). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-FANCA/Faca Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03662)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03662 is reactive to FANCA in Human

Applications

A03662 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

162.775kDa

Background of FACA/FANCA

FANCA (also called Protein FACA or Fanconi anemia group A protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCA may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, and FANCL. The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Diseases associated with FANCA include Fanconi anemia complementation group a and Congenital Hypoplastic Anemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:15,000 - 1:60,000
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of FANCA expression in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection.. FANCA was detected using rabbit anti-FANCA Antigen Affinity purified polyclonal antibody (Catalog # A03662). Modified from Smogorzewska et al (2007) Cell 129, 289-301.

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Western blot analysis of FANCA expression in HELA whole cell lysates (lane 1). FANCA at 133KD was detected using rabbit anti-FANCA Antigen Affinity purified polyclonal antibody (Catalog # A03662) at 1:1500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For FANCA (Source: Uniprot.org, NCBI)

Alternative Names

FA; FA1; FAA; FAAMGC75158; FACA; FACAFanconi anemia group A protein; FAH; FA-H; FANCA; FANCH; Fanconi anemia, complementation group A; Fanconi anemia, complementation group H; Fanconi anemia, type 1; Protein FACA FANCA FA, FA-H, FA1, FAA, FACA, FAH, FANCH FA complementation group A Fanconi anemia group A protein|Fanconi anemia complementation group A|Fanconi anemia, complementation group H|Fanconi anemia, type 1

For more info on FANCA, check out the FANCA Infographic

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In this infographic, you will see the following information for FANCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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