Click image to see more details
Product Info Summary
| SKU: | A00563 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-FANCD2 Antibody
SKU/Catalog Number
A00563
Size
100μl
Form
Liquid
Description
Boster Bio Anti-FANCD2 Antibody catalog # A00563. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FANCD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00563)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FANCD2. AA range:188-237
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00563 is reactive to FANCD2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
164.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00563 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using FANCD2 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FANCD2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-FANCD2 Antibody (A00563)
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Customer Q&As
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7 Customer Q&As for Anti-FANCD2 Antibody
Question
Does A00563 anti-FANCD2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
You can see on the product datasheet, A00563 anti-FANCD2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-23
Question
Is a blocking peptide available for product anti-FANCD2 antibody (A00563)?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We do provide the blocking peptide for product anti-FANCD2 antibody (A00563). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-13
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-FANCD2 antibody A00563. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-26
Question
Do you have a BSA free version of anti-FANCD2 antibody A00563 available?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FANCD2 antibody A00563 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-02
Question
I see that the anti-FANCD2 antibody A00563 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-FANCD2 antibody A00563. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-25
Question
Would anti-FANCD2 antibody A00563 work on horse WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
Our lab technicians have not validated anti-FANCD2 antibody A00563 on horse. You can run a BLAST between horse and the immunogen sequence of anti-FANCD2 antibody A00563 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-09
