Anti-Fanconi anemia group G protein FANCG Antibody

Product Name

Anti-Fanconi anemia group G protein FANCG Antibody

View all FANCG Antibodies

SKU/Catalog Number

A03129

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Fanconi anemia group G protein FANCG Antibody (Catalog # A03129). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Fanconi anemia group G protein FANCG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03129)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region near amino acids 1-25 of human FANCG protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03129 is reactive to FANCG in Human, Mouse

Applications

A03129 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

68.554kDa

Background of FANCG

FANCG (also called Protein FACG or DNA-repair protein XRCC9) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCG may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. This protein may also function as a tumor suppressor gene. FANCG belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. FANCG is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is highly expressed in testis and thymus and is also found in lymphoblasts. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:2,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Affinity Purified anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue.

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Affinity Purified anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue.

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Figure 1. Western blot analysis of FANCG using anti-FANCG antibody (A03129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCG antigen affinity purified polyclonal antibody (Catalog # A03129) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

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Figure 3. Western blot analysis of FANCG using anti-FANCG antibody (A03129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCG antigen affinity purified polyclonal antibody (Catalog # A03129) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

Click image to see more details

Figure 5. Western blot analysis of FANCG using anti-FANCG antibody (A03129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCG antigen affinity purified polyclonal antibody (Catalog # A03129) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.

Gene/Protein Information For FANCG (Source: Uniprot.org, NCBI)

Alternative Names

DNA repair protein XRCC9; FAG; Fanconi anemia group G protein; Fanconi anemia, complementation group G; Protein FACG; X-ray repair, complementing defective, in Chinese hamster, 9; XRCC9X-ray repair complementing defective repair in Chinese hamster cells 9 FANCG FAG, XRCC9 FA complementation group G Fanconi anemia group G protein|DNA repair protein XRCC9|Fanconi anemia complementation group G|X-ray repair complementing defective repair in Chinese hamster cells 9|X-ray repair, complementing defective, in Chinese hamster, 9|truncated Fanconi anemia group G protein

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