Product Info Summary
SKU: | A03129 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Fanconi anemia group G protein FANCG Antibody
SKU/Catalog Number
A03129
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Fanconi anemia group G protein FANCG Antibody (Catalog # A03129). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-Fanconi anemia group G protein FANCG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03129)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region near amino acids 1-25 of human FANCG protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03129 is reactive to FANCG in Human, Mouse
Applications
A03129 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
68.554kDa
Background of FANCG
FANCG (also called Protein FACG or DNA-repair protein XRCC9) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCG may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. This protein may also function as a tumor suppressor gene. FANCG belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. FANCG is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is highly expressed in testis and thymus and is also found in lymphoblasts. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:2,000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Affinity Purified anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue.
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Affinity Purified anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue.
Click image to see more details
Figure 1. Western blot analysis of FANCG using anti-FANCG antibody (A03129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCG antigen affinity purified polyclonal antibody (Catalog # A03129) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Click image to see more details
Figure 3. Western blot analysis of FANCG using anti-FANCG antibody (A03129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCG antigen affinity purified polyclonal antibody (Catalog # A03129) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Click image to see more details
Figure 5. Western blot analysis of FANCG using anti-FANCG antibody (A03129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCG antigen affinity purified polyclonal antibody (Catalog # A03129) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For FANCG (Source: Uniprot.org, NCBI)
Gene Name
FANCG
Full Name
Fanconi anemia group G protein
Weight
68.554kDa
Alternative Names
DNA repair protein XRCC9; FAG; Fanconi anemia group G protein; Fanconi anemia, complementation group G; Protein FACG; X-ray repair, complementing defective, in Chinese hamster, 9; XRCC9X-ray repair complementing defective repair in Chinese hamster cells 9 FANCG FAG, XRCC9 FA complementation group G Fanconi anemia group G protein|DNA repair protein XRCC9|Fanconi anemia complementation group G|X-ray repair complementing defective repair in Chinese hamster cells 9|X-ray repair, complementing defective, in Chinese hamster, 9|truncated Fanconi anemia group G protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FANCG, check out the FANCG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FANCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fanconi anemia group G protein FANCG Antibody (A03129)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Fanconi anemia group G protein FANCG Antibody
Question
Is this A03129 anti-FANCG antibody reactive to the isotypes of FANCG?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The immunogen of A03129 anti-FANCG antibody is This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-12 of human FANCG protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-13
Question
Please see the WB image, lot number and protocol we used for kidney uterus using anti-FANCG antibody A03129. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney uterus using anti-FANCG antibody A03129. Let me know if you need anything else.
D. Krishna
Verified customer
Asked: 2019-05-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-FANCG antibody A03129 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
The anti-FANCG antibody (A03129) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-05
Question
I was wanting to use your anti-FANCG antibody for WB for human kidney uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney uterus identification?
H. Brown
Verified customer
Asked: 2018-01-17
Answer
As indicated on the product datasheet, A03129 anti-FANCG antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-17