Anti-Fascin/FSCN1 Antibody Picoband™

Product Name

Anti-Fascin/FSCN1 Antibody Picoband™

View all Fascin Antibodies

SKU/Catalog Number

PB9592

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fascin/FSCN1 Antibody Picoband™ catalog # PB9592. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fascin/FSCN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9592)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9592 is reactive to FSCN1 in Human, Mouse, Rat

Applications

PB9592 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

54.53kDa

Background of Fascin

Fascin is an actin cross-linking protein. The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin (from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Fascin/FSCN1 using anti-Fascin/FSCN1 antibody (PB9592).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fascin/FSCN1 antigen affinity purified polyclonal antibody (Catalog # PB9592) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fascin/FSCN1 at approximately 55 kDa. The expected band size for Fascin/FSCN1 is at 55 kDa.

Gene/Protein Information For FSCN1 (Source: Uniprot.org, NCBI)

Alternative Names

FAN1; FAN1,55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus); Fascin; FLJ38511; FSCN1; HSN; p55actin bundling protein; singed (Drosophila)-like (sea urchin fascin homolog like); singed-like (fascin homolog, sea urchin); Singed-like Protein; SNL; SNLfascin FSCN1 FAN1, HSN, SNL, p55 fascin actin-bundling protein 1 fascin|55 kDa actin-bundling protein|epididymis secretory sperm binding protein|fascin homolog 1, actin-bundling protein|singed-like (fascin homolog, sea urchin)

For more info on FSCN1, check out the FSCN1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FSCN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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