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Product Info Summary
| SKU: | M05118 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FDFT1 Monoclonal Antibody
SKU/Catalog Number
M05118
BM5781 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FDFT1 Monoclonal Antibody catalog # M05118. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FDFT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05118)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFHH-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FDFT1 Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway.
Reactive Species
M05118 is reactive to FDFT1 in Human, Mouse, Rat
Observed Molecular Weight
48 kDa
Calculated molecular weight
48.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05118 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of FDFT1 expression in (1) HepG2 cell lysate; (2) RAW264.7 cell lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Specific Publications For Anti-FDFT1 Monoclonal Antibody (M05118)
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4 Customer Q&As for Anti-FDFT1 Monoclonal Antibody
Question
Will M05118 anti-FDFT1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
You can see on the product datasheet, M05118 anti-FDFT1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-FDFT1 Monoclonal antibody M05118. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-FDFT1 Monoclonal antibody M05118 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
F. Jackson
Verified customer
Asked: 2019-01-16
Answer
The anti-FDFT1 Monoclonal antibody (M05118) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-16
Question
Is there a BSA free version of anti-FDFT1 Monoclonal antibody M05118 available?
M. Jones
Verified customer
Asked: 2017-06-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FDFT1 Monoclonal antibody M05118 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-21
