Anti-FENS1/WDFY1 Antibody Picoband™

Product Name

Anti-FENS1/WDFY1 Antibody Picoband™

View all FENS1 Antibodies

SKU/Catalog Number

A10318-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FENS1/WDFY1 Antibody Picoband™ catalog # A10318-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-FENS1/WDFY1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10318-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FENS1/WDFY1 recombinant protein (Position: H65-D394).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10318-3 is reactive to WDFY1 in Human, Mouse, Rat

Applications

A10318-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

46.324kDa

Background of FENS1

The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FENS1/WDFY1 using anti-FENS1/WDFY1 antibody (A10318-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FENS1/WDFY1 antigen affinity purified polyclonal antibody (Catalog # A10318-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FENS1/WDFY1 at approximately 46 kDa. The expected band size for FENS1/WDFY1 is at 46 kDa.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-FENS1/WDFY1 antibody (A10318-3).
Overlay histogram showing HL-60 cells stained with A10318-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FENS1/WDFY1 Antibody (A10318-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For WDFY1 (Source: Uniprot.org, NCBI)

Alternative Names

FENS-1Zinc finger FYVE domain-containing protein 17; KIAA1435; Phosphoinositide-binding protein 1; WD repeat and FYVE domain containing 1; WD repeat and FYVE domain-containing protein 1; WD40 and FYVE domain containing 1; WDF1WD40- and FYVE domain-containing protein 1; ZFYVE17phosphoinositide-binding protein SR1 WDFY1 FENS-1, FENS1, WDF1, ZFYVE17 WD repeat and FYVE domain containing 1 WD repeat and FYVE domain-containing protein 1|FYVE domain-containing protein localized to endosomes 1|WD40- and FYVE domain-containing protein 1|phosphoinositide-binding protein SR1|zinc finger FYVE domain-containing protein 17

For more info on WDFY1, check out the WDFY1 Infographic

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In this infographic, you will see the following information for WDFY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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