Anti-FFR/VPS51 Antibody Picoband™

Product Name

Anti-FFR/VPS51 Antibody Picoband™

View all VPS51 Antibodies

SKU/Catalog Number

A08518-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FFR/VPS51 Antibody Picoband™ catalog # A08518-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-FFR/VPS51 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08518-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FFR/VPS51 recombinant protein (Position: K38-R781).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08518-1 is reactive to VPS51 in Human

Applications

A08518-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

86 kDa

Calculated molecular weight

86.042kDa

Background of VPS51

This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FFR/VPS51 using anti-FFR/VPS51 antibody (A08518-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human T-47D whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFR/VPS51 antigen affinity purified polyclonal antibody (Catalog # A08518-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FFR/VPS51 at approximately 86 kDa. The expected band size for FFR/VPS51 is at 86 kDa.

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Figure 2. Flow Cytometry analysis of U20S cells using anti-FFR/VPS51 antibody (A08518-1).
Overlay histogram showing U20S cells stained with A08518-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FFR/VPS51 Antibody (A08518-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For VPS51 (Source: Uniprot.org, NCBI)

Alternative Names

ANG2; ANG3; C11orf2; C11orf3; chromosome 11 open reading frame 2; FFR; protein fat-free homolog; vacuolar protein sorting 51 homolog (S. cerevisiae) VPS51 ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13 VPS51 subunit of GARP complex vacuolar protein sorting-associated protein 51 homolog|VPS51, GARP complex subunit|another new gene 2 protein|protein fat-free homolog|vacuolar protein sorting 51 homolog

For more info on VPS51, check out the VPS51 Infographic

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In this infographic, you will see the following information for VPS51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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